What Is Trophoblast? A Combination of Criteria Define Human First-Trimester Trophoblast

被引：232

作者：

Lee, Cheryl Q. E. ^{[1
,2
,3
]}

Gardner, Lucy ^{[1
,2
]}

Turco, Margherita ^{[1
,2
]}

Zhao, Nancy ^{[1
]}

Murray, Matthew J. ^{[1
]}

Coleman, Nicholas ^{[1
]}

Rossant, Janet ^{[3
,4
]}

Hemberger, Myriam ^{[2
,5
]}

Moffett, Ashley ^{[1
,2
]}

机构：

[1] Univ Cambridge, Dept Pathol, Tennis Court Rd, Cambridge CB2 1QP, England

[2] Univ Cambridge, Ctr Trophoblast Res, Cambridge CB2 3EG, England

[3] Hosp Sick Children, Program Dev & Stem Cell Biol, Toronto, ON M5G 0A4, Canada

[4] Univ Toronto, Dept Mol Genet, Toronto, ON M5G 0A4, Canada

[5] Babraham Inst, Epigenet Programme, Cambridge CB22 3AT, England

来源：

STEM CELL REPORTS | 2016年 / 6卷 / 02期

基金：

加拿大健康研究院; 英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

EMBRYONIC STEM-CELLS; PLACENTAL LEUCINE AMINOPEPTIDASE/OXYTOCINASE; MAJOR HISTOCOMPATIBILITY ANTIGENS; HUMAN MONOCLONAL-ANTIBODIES; CARCINOMA IN-SITU; EXTRAVILLOUS TROPHOBLAST; C19MC MICRORNAS; RT-PCR; EXPRESSION; DIFFERENTIATION;

D O I：

10.1016/j.stemcr.2016.01.006

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Controversy surrounds reports describing the derivation of human trophoblast cells from placentas and embryonic stem cells (ESC), partly due to the difficulty in identifying markers that define cells as belonging to the trophoblast lineage. We have selected criteria that are characteristic of primary first-trimester trophoblast: a set of protein markers, HLA class I profile, methylation of ELF5, and expression of microRNAs (miRNAs) from the chromosome 19 miRNA cluster (C19MC). We tested these criteria on cells previously reported to show some phenotypic characteristics of trophoblast: bone morphogenetic protein (BMP)-treated human ESC and 2102Ep, an embryonal carcinoma cell line. Both cell types only show some, but not all, of the four trophoblast criteria. Thus, BMP-treated human ESC have not fully differentiated to trophoblast. Our study identifies a robust panel, including both protein and non-protein-coding markers that, in combination, can be used to reliably define cells as characteristic of early trophoblast.

引用

页码：257 / 272

页数：16

共 80 条

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