Domains in the XPA protein important in its role as a processivity factor

被引:27
作者
Bartels, Claudine L.
Lambert, Muriel W. [1 ]
机构
[1] Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Pathol, Newark, NJ 07103 USA
[2] Univ Med & Dent New Jersey, New Jersey Med Sch, Lab Med, Newark, NJ 07103 USA
[3] Univ Med & Dent New Jersey Grad Sch Biomed Sci, Newark, NJ 07103 USA
关键词
xeroderma pigmentosum; XPA protein; DNA repair; processive mechanism of action;
D O I
10.1016/j.bbrc.2007.02.125
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
XPA is a protein essential for nucleotide excision repair (NER) where it is thought to function in damage recognition/verification. We have proposed an additional role, that of a processivity factor, conferring a processive mechanism of action on XPF and XPG, the endonucleases. involved in NER. The present study was undertaken to examine the domain(s) in the XPA gene that are important for the ability of the XPA protein to function as a processivity factor. Using site-directed mutagenesis, mutations were created in several of the exons of XPA and mutant XPA proteins produced. The results showed that the DNA binding domain of XPA is critical for its ability to act as a processivity factor. Mutations in both the zinc finger motif and the large basic cleft in this domain eliminated the ability of XPA to confer a processive mechanism of action on the endonucleases involved in NER. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:219 / 225
页数:7
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