The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication

被引：20

作者：

Zhao, Wan ^{[1
,2
]}

Zhu, Junjie ^{[1
,2
]}

Lu, Hong ^{[1
,2
]}

Zhu, Jiaming ^{[1
,2
]}

Jiang, Fei ^{[1
,2
]}

Wang, Wei ^{[1
,2
]}

Luo, Lan ^{[1
,2
]}

Kang, Le ^{[1
,2
]}

Cui, Feng ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, State Key Lab Integrated Management Pest Insects, Inst Zool, Beijing 100101, Peoples R China

[2] Univ Chinese Acad Sci, CAS Ctr Excellence Biot Interact, Beijing 100049, Peoples R China

来源：

PROTEIN & CELL | 2022年 / 13卷 / 05期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

rice stripe virus; nucleocapsid protein; nuclear localization; importin α YY1;

D O I：

10.1007/s13238-021-00822-1

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Rice stripe virus (RSV) transmitted by the small brown planthopper causes severe rice yield losses in Asian countries. Although viral nuclear entry promotes viral replication in host cells, whether this phenomenon occurs in vector cells remains unknown. Therefore, in this study, we systematically evaluated the presence and roles of RSV in the nuclei of vector insect cells. We observed that the nucleocapsid protein (NP) and viral genomic RNAs were partially transported into vector cell nuclei by utilizing the importin alpha nuclear transport system. When blocking NP nuclear localization, cytoplasmic RSV accumulation significantly increased. In the vector cell nuclei, NP bound the transcription factor YY1 and affected its positive regulation to FAIM. Subsequently, decreased FAIM expression triggered an antiviral caspase-dependent apoptotic reaction. Our results reveal that viral nuclear entry induces completely different immune effects in vector and host cells, providing new insights into the balance between viral load and the immunity pressure in vector insects.

引用

页码：360 / 378

页数：19

共 60 条

[31] Hepatitis C virus core protein inhibits Fas- and tumor necrosis factor alpha-mediated apoptosis via NF-κB activation [J].