Protein quantification in the presence of poly(ethylene glycol) and dextran using the Bradford method

被引：85

作者：

Barbosa, Helder ^{[1
]}

Slater, Nigel K. H. ^{[2
]}

Marcos, Joao C. ^{[1
]}

机构：

[1] Univ Minho, Ctr Chem, P-4710057 Braga, Portugal

[2] Univ Cambridge, Dept Chem Engn & Biotechnol, Cambridge CB2 3RA, England

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 395卷 / 01期

关键词：

PRECIPITATION; SYSTEMS;

D O I：

10.1016/j.ab.2009.07.045

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Some experimental methodologies require the quantification of protein in the presence of polymers like poly(ethylene glycol) (PEG) and dextran (DEX). In the aqueous two-phase system (ATPS) extraction of biomolecules, the interference of these phase-forming polymers on the Bradford quantification assay is commonly recognized. However, how these polymers interfere has not been reported hitherto. In this study we show that while dextran concentrations of 20% (w/w) can be used without error, loss of accuracy occurs for solutions with PEG concentrations > 10% (w/w). Above this value a substantial decrease on the assay sensitivity is observed. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：108 / 110

页数：3

共 12 条

[1] Affinity partitioning of a Cellulomonas fimi β-mannanase with a mannan-binding module in galactomannan/starch aqueous two-phase system [J].