A positive feedback loop promotes transcription surge that jump-starts Salmonella virulence circuit

被引：112

作者：

Shin, Dongwoo ^{[1
]}

Lee, Eun-Jin ^{[1
]}

Huang, Henry ^{[1
]}

Groisman, Eduardo A. ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Dept Mol Microbiol, Howard Hughes Med Inst, St Louis, MO 63110 USA

来源：

SCIENCE | 2006年 / 314卷 / 5805期

关键词：

D O I：

10.1126/science.1134930

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The PhoP/ PhoQ two-component system is a master regulator of Salmonella pathogenicity. Here we report that induction of the PhoP/ PhoQ system results in an initial surge of PhoP phosphorylation; the occupancy of target promoters by the PhoP protein; and the transcription of PhoP-activated genes, which then subsides to reach new steady-state levels. This surge in PhoP activity is due to PhoP positively activating its own transcription, because a strain constitutively expressing the PhoP protein attained steady-state levels of activation asymptotically, without the surge. The strain constitutively expressing the PhoP protein was attenuated for virulence in mice, demonstrating that the surge conferred by PhoP's positive feedback loop is necessary to jump-start Salmonella's virulence program.

引用

页码：1607 / 1609

页数：3

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