Telomerase and hTERT: Can they serve as markers for gastric cancer diagnosis?

被引：2

作者：

Cheng, Yong-Bo ^{[1
]}

Guo, Li-Ping ^{[2
]}

Yao, Ping ^{[1
]}

Ning, Xiao-Yan ^{[2
]}

Aerken, Gulimire ^{[1
]}

Fang, Dian-Chun ^{[2
]}

机构：

[1] Xinjiang Med Univ, Affiliated Hosp 1, Dept Gastroenterol, Urumqi 830054, Peoples R China

[2] Third Mil Med Univ, Southwest Hosp, Dept Gastroenterol, Chongqing 400038, Peoples R China

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2014年 / 20卷 / 21期

基金：

中国国家自然科学基金;

关键词：

Gastric cancer; Telomerase; Human telomerase reverse transcriptase; Normal human gastric mucosal epithelial cell; Normal human gastric mucosal fibroblast; TRANSCRIPTASE MESSENGER-RNA; REVERSE-TRANSCRIPTASE; TUMOR-MARKER; EXPRESSION; ACTIVATION; LESIONS; PLASMA; TARGET;

D O I：

10.3748/wjg.v20.i21.6615

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To investigate telomerase activity and human telomerase reverse transcriptase (hTERT) expression in normal human gastric mucosal epithelial cells (nhGMECs) and fibroblasts (nhGMFs). METHODS: nhGMECs and nhGMFs were isolated and cultured from specimens obtained during routine surgery for bleeding peptic ulcer. Telomerase activity in nhGMFs, nhGMECs, and the tumor cell lines BGC-823, SGC-7901 and MKN-28 cells was analyzed using the telomeric repeat amplification protocol assay. hTERT protein was determined in nhGMECs, nhGMFs, BGC-823, SGC-7901 and MKN-28 cells by indirect immunofluorescence. RESULTS: A similar level of telomerase activity was observed in nhGMECs, nhGMFs and BGC-823, SGC-7901, MKN-28 cell lines. Positive hTERT immunostaining was detected in nhGMECs, nhGMFs, BGC-823, SGC-7901 and MKN-28 cell lines. CONCLUSION: The use of telomerase or hTERT as diagnostic markers for gastric cancer may require further studies. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.

引用

页码：6615 / 6619

页数：5

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