Measurement of 3-nitrotyrosine and 5-nitro-γ-tocopherol by high-performance liquid chromatography with electrochemical detection

被引：59
作者：
Hensley, K [1 ]
Williamson, KS [1 ]
Floyd, RA [1 ]
机构：
[1] Oklahoma Med Res Fdn, Free Rad Biol & Aging Res Program, Oklahoma City, OK 73104 USA
来源：
FREE RADICAL BIOLOGY AND MEDICINE | 2000年 / 28卷 / 04期
关键词：
free radical; nitric oxide; nitrotyrosine; nitrotocopherol; HPLC; electrochemical; review;
D O I：
10.1016/S0891-5849(00)00155-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Nitric oxide (NO) is a lipophilic gaseous molecule synthesized by the enzymatic oxidation of L-arginine. During periods of inflammation, phagocytic cells generate copious quantities of NO and other reactive oxygen species. The combination of NO with other reactive oxygen species promotes nitration of ambient biomolecules, including protein tyrosine residues and membrane-localized gamma-tocopherol. The oxidative chemistry of NO and derived redox congeners is reviewed. Techniques are described for the determination of 3-nitro-tyrosine and 5-nitro-gamma-tocopherol in biological samples using high-performance liquid chromatography with electrochemical detection. (C) 2000 Elsevier Science Inc.
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页码：520 / 528
页数：9
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