Teaching Undergraduates LC-MS/MS Theory and Operation via Multiple Reaction Monitoring (MRM) Method Development

被引：18

作者：

Betts, Thomas A. ^{[1
]}

Palkendo, Julie A. ^{[1
]}

机构：

[1] Kutztown State Univ, Dept Phys Sci, Kutztown, PA 19530 USA

来源：

JOURNAL OF CHEMICAL EDUCATION | 2018年 / 95卷 / 06期

关键词：

Upper-Division Undergraduate; Analytical Chemistry; Laboratory Instruction; Hands-On Learning/Manipulatives; Chromatography; Mass Spectrometry; Consumer Chemistry; MASS-SPECTROMETRY; VANILLA EXTRACT; VALIDATION; COUMARIN;

D O I：

10.1021/acs.jchemed.7b00914

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This laboratory experiment employs an innovative approach to introduce undergraduates to liquid chromatography tandem mass spectrometry (LC-MS/MS) by developing a method based on multiple reaction monitoring (MRM). During this qualitative investigation, students operated a triple quadrupole mass spectrometer in the MS scan and product ion scan modes to determine precursor and product ions, respectively, for a vanillin, ethyl vanillin, and coumarin standard mixture. After identifying precursor -> product ion transitions, students conducted a 2 min liquid chromatography run in MRM mode. Students were astonished by the speed, selectivity, and sensitivity of the LC-MS/MS method compared to that of a liquid chromatograph detection.

引用

页码：1035 / 1039

页数：5

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