Detection of Pectinatus spp. by PCR using 16S-23S rDNA spacer regions

被引：0

作者：

Motoyama, Y ^{[1
]}

Ogata, T ^{[1
]}

机构：

[1] Asahi Breweries Ltd, Brewing Res & Dev Lab, Moriya, Ibaraki 3020106, Japan

来源：

JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS | 2000年 / 58卷 / 01期

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A polymerase chain reaction (PCR) method for the detection of Pectinatus cerevisiiphilus and P. frisingensis was developed. The 16S rRNA gene (rDNA) and the spacer regions between the 16S rDNA and the 23S rDNA genes were used for the PCR reaction. The species-specific sequences in the spacer region between the 16S rDNA and the 23S rDNA genes were selected for use as PCR primers. The method developed in this study was rapid and sensitive. Furthermore, this method allowed identification at the species level, even between very closely related species, such as P. cerevisiiphilus and P. frisingensis.

引用

页码：4 / 7

页数：4

共 50 条

[31] Comprehensive detection of bacterial populations by PCR amplification of the 16S-23S rRNA spacer region
González, N
Romero, J
Espejo, RT
JOURNAL OF MICROBIOLOGICAL METHODS, 2003, 55 (01) : 91 - 97
[32] Development of a novel triplex PCR assay for the detection and differentiation of thermophilic species of Campylobacter using 16S-23S rDNA internal transcribed spacer (ITS) region
Khan, I. U. H.
Edge, T. A.
JOURNAL OF APPLIED MICROBIOLOGY, 2007, 103 (06) : 2561 - 2569
[33] Length polymorphisms for intergenic spacer regions of 16S-23S rDNA in members of the new hydrogen-producing bacteria
李永峰
徐菁利
杨传平
任南琪
Journal of Harbin Institute of Technology(New series), 2007, (05) : 691 - 694
[34] The genetic characterization of Pseudomonas syringae pv. tagetis based on the 16S-23S rDNA intergenic spacer regions
Kong, H
Blackwood, C
Buyer, JS
Gulya, TJ
Lydon, J
BIOLOGICAL CONTROL, 2005, 32 (03) : 356 - 362
[35] Molecular differentiation of bacteria by PCR amplification of the 16S-23S rRNA spacer
Scheinert, P
Krausse, R
Ullmann, U
Soller, R
Krupp, G
JOURNAL OF MICROBIOLOGICAL METHODS, 1996, 26 (1-2) : 103 - 117
[36] PCR detection of Klebsiella pneumoniae in infant formula based on 16S-23S internal transcribed spacer
Liu, Yin
Liu, Chao
Zheng, Wenjie
Zhang, Xia
Yu, Jia
Gao, Qili
Hou, Yanmei
Huang, Xitai
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2008, 125 (03) : 230 - 235
[37] A rapid PCR procedure for the specific identification of Lactobacillus sanfranciscensis, based on the 16S-23S intergenic spacer regions
Valcheva, R.
Kabadjova, P.
Rachman, C.
Ivanova, I.
Onno, B.
Prevost, H.
Dousset, X.
JOURNAL OF APPLIED MICROBIOLOGY, 2007, 102 (01) : 290 - 302
[38] Development of a PCR assay based on the 16S-23S rDNA internal transcribed spacer for identification of strictly anaerobic bacterium Zymophilus
Felsberg, Jurgen
Jelinkova, Marketa
Kubizniakova, Petra
Matoulkova, Dagmar
ANAEROBE, 2015, 33 : 85 - 89
[39] Utility of internally transcribed 16S-23S rDNA spacer regions for the definition of Pseudomonas stutzeri genomovars and other Pseudomonas species
Guasp, C
Moore, ERB
Lalucat, J
Bennasar, A
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 : 1629 - 1639
[40] Re-identification of Gluconobacter strains based on restriction analysis of 16S-23S rDNA internal transcribed spacer regions
Yukphan, P
Malimas, T
Takahashi, M
Potacharoen, W
Busabun, T
Tanasupawat, S
Nakagawa, Y
Tanticharoen, M
Yamada, Y
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2004, 50 (04) : 189 - 195

← 1 2 3 4 5 →