Highly Effective and Noninvasive Near-Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min

被引：291

作者：

Li, Mu ^{[1
]}

Li, Liqian ^{[1
]}

Su, Kun ^{[1
]}

Liu, Xiangmei ^{[1
]}

Zhang, Tianjin ^{[1
]}

Liang, Yanqin ^{[2
]}

Jing, Doudou ^{[3
]}

Yang, Xianjin ^{[2
]}

Zheng, Dong ^{[3
]}

Cui, Zhenduo ^{[2
]}

Li, Zhaoyang ^{[2
]}

Zhu, Shengli ^{[2
]}

Yeung, Kelvin Wai Kwok ^{[4
]}

Zheng, Yufeng ^{[5
,6
]}

Wang, Xianbao ^{[1
]}

Wu, Shuilin ^{[1
,2
]}

机构：

[1] Hubei Univ, Sch Mat Sci & Engn, Key Lab Green Preparat & Applicat Funct Mat, Hubei Key Lab Polymer Mat,Minist Educ, Wuhan 430062, Hubei, Peoples R China

[2] Tianjin Univ, Minist Educ China, Sch Mat Sci & Engn, Key Lab Adv Ceram & Machining Technol, Tianjin 300072, Peoples R China

[3] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Orthopaed, Wuhan 430022, Hubei, Peoples R China

[4] Univ Hong Kong, Li Ka Shing Fac Med, Dept Orthopaed & Traumatol, Pokfulam, Hong Kong 999077, Peoples R China

[5] Peking Univ, Coll Engn, State Key Lab Turbulence & Complex Syst, Beijing 100871, Peoples R China

[6] Peking Univ, Coll Engn, Dept Mat Sci & Engn, Beijing 100871, Peoples R China

来源：

ADVANCED SCIENCE | 2019年 / 6卷 / 17期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

antibacterial; biofilms; IR780; MoS2; phototherapy; MOS2; NANOPARTICLES; GRAPHENE; INACTIVATION; BIOMATERIALS; MECHANISMS; NANOSHEETS; TRACKING; ADHESION; DELIVERY;

D O I：

10.1002/advs.201900599

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Biofilms have been related to the persistence of infections on medical implants, and these cannot be eradicated because of the resistance of biofilm structures. Therefore, a biocompatible phototherapeutic system is developed composed of MoS2, IR780 photosensitizer, and arginine-glycine-aspartic acid-cysteine (RGDC) to safely eradicate biofilms on titanium implants within 20 min. The magnetron-sputtered MoS2 film possesses excellent photothermal properties, and IR780 can produce reactive oxygen species (ROS) with the irradiation of near-infrared (NIR, lambda = 700-1100 nm) light. Consequently, the combination of photothermal therapy (PTT) and photodynamic therapy (PDT), assisted by glutathione oxidation accelerated by NIR light, can provide synergistic and rapid killing of bacteria, i.e., 98.99 +/- 0.42% eradication ratio against a Staphylococcus aureus biofilm in vivo within 20 min, which is much greater than that of PTT or PDT alone. With the assistance of ROS, the permeability of damaged bacterial membranes increases, and the damaged bacterial membranes become more sensitive to heat, thus accelerating the leakage of proteins from the bacteria. In addition, RGDC can provide excellent biosafety and osteoconductivity, which is confirmed by in vivo animal experiments.

引用

页数：16

共 58 条

[1]

Acerce M, 2015, NAT NANOTECHNOL, V10, P313, DOI [10.1038/nnano.2015.40, 10.1038/NNANO.2015.40]

[2] Hydrophobic Ice Confined between Graphene and MoS2 [J].