Long Noncoding RNAs and Messenger RNAs Expression Profiles Potentially Regulated by ZBTB7A in Nasopharyngeal Carcinoma

被引:3
|
作者
Liu, Fei [1 ]
Wei, Jiazhang [2 ]
Hao, Yanrong [3 ]
Tang, Fengzhu [2 ]
Jiao, Wei [1 ]
Qu, Shenhong [2 ]
He, Ning [2 ]
Cai, Yonglin [4 ]
Lan, Jiao [1 ]
Yang, Yong [2 ]
Wang, Yongli [2 ]
Li, Min [2 ]
Weng, Jingjin [2 ]
Li, Bing [2 ]
Lu, Jinlong [2 ]
Han, Xing [2 ]
机构
[1] Peoples Hosp Guangxi Zhuang Autonomous Reg, Res Ctr Med Sci, Nanning 530021, Peoples R China
[2] Peoples Hosp Guangxi Zhuang Autonomous Reg, Dept Otolaryngol & Head & Neck, Nanning 530021, Peoples R China
[3] Peoples Hosp Guangxi Zhuang Autonomous Reg, Canc Ctr, Nanning 530021, Peoples R China
[4] Wuzhou Red Cross Hosp, Key Lab Nasopharyngeal Carcinoma Etiol & Mol Mech, Wuzhou 543002, Peoples R China
关键词
BARR-VIRUS DNA; PROMOTES CELL-PROLIFERATION; TUMOR-SUPPRESSOR; POOR-PROGNOSIS; GLUCOSE-METABOLISM; DOWN-REGULATION; LNCRNA ANRIL; CANCER; INVASION; OVEREXPRESSION;
D O I
10.1155/2019/7246491
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Our previous studies showed that ZBTB7A played an important role in promoting nasopharyngeal carcinoma (NPC) progression. However, molecular mechanisms of different levels of ZBTB7A are still unclear. It is necessary to search molecular markers which are closely connected with ZBTB7A. We selected NPC sublines CNE2 with stably transfecting empty plasmid (negative control, NC) and short hair RNA (shRNA) plasmid targeting ZBTB7A as research objectives. Microarray was used to screen differentially expressed long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) via shRNA-CNE2 versus NC-CNE2. Quantitative PCR (qPCR) was used to validate lncRNAs and mRNAs from the sublines, chronic rhinitis, and NPC tissues. Bioinformatics was used to analyze regulatory pathways which were connected with ZBTB7A. The 1501 lncRNAs (long noncoding RNAs) and 1275 differentially expressed mRNAs were upregulated or downregulated over 2-fold. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the upregulated or downregulated carbohydrate and lipid metabolisms probably involved in carcinogenicity of shRNA-CNE2 (P-value cut-off was 0.05). In order to find the molecular mechanisms of ZBTB7A, we validated 12 differentially expressed lncRNAs and their nearby mRNAs by qPCR. Most of the differentially expressed mRNAs are closely connected with carbohydrate and lipid metabolisms in multiply cancers. Furthermore, part of them were validated in NPC and rhinitis tissues by qPCR. As a result, NR_047538, ENST00000442852, and fatty acid synthase (FASN) were closely associated with NPC. ZBTB7A had a positive association with NR_047538 and negative associations with ENST00000442852 and FASN. The results probably provide novel candidate biomarkers for NPC progression with different levels of ZBTB7A.
引用
收藏
页数:16
相关论文
共 50 条
  • [31] Long noncoding RNAs expression in gastric cancer
    Esfandi, Farbod
    Taheri, Mohammad
    Oskooei, Vahid Kholghi
    Ghafouri-Fard, Soudeh
    JOURNAL OF CELLULAR BIOCHEMISTRY, 2019, 120 (08) : 13802 - 13809
  • [32] Long Noncoding RNAs as Enhancers of Gene Expression
    Orom, U. A.
    Derrien, T.
    Guigo, R.
    Shiekhattar, R.
    NUCLEAR ORGANIZATION AND FUNCTION, 2010, 75 : 325 - 331
  • [33] Dysregulated Expression of Long Noncoding RNAs in Endometriosis
    Zhang, Xue-ying
    Zheng, Lian-wen
    Li, Chun-jin
    Xu, Ying
    Zhou, Xu
    Fu, Lu-lu
    Li, Dan-dan
    Sun, Li-ting
    Zhang, Di
    Cui, Man-hua
    CRITICAL REVIEWS IN EUKARYOTIC GENE EXPRESSION, 2019, 29 (02): : 113 - 121
  • [34] Altered Expression of Long Noncoding and Messenger RNAs in Diabetic Nephropathy following Treatment with Rosiglitazone
    Zhang, Liwen
    Zhou, Ying
    Zhou, Fangfang
    Yu, Xialian
    Liu, Jian
    Liu, Yunzi
    Zhu, Yufei
    Wang, Weiming
    Chen, Nan
    BIOMED RESEARCH INTERNATIONAL, 2020, 2020
  • [35] Crosstalk between prognostic long noncoding RNAs and messenger RNAs as transcriptional hallmarks in gastric cancer
    Zhang, Jian
    Yuan, Yujie
    Wei, Zhewei
    Ren, Jianwei
    Hou, Xun
    Yang, Dongjie
    Cai, Sirong
    Chen, Chuangqi
    Tan, Min
    Chen, George Gong
    Wu, Kaiming
    He, Yulong
    EPIGENOMICS, 2018, 10 (04) : 433 - 443
  • [36] Differences in Zbtb7a expression cause heterogeneous changes in human nasopharyngeal carcinoma CNE3 sublines
    Liu, Fei
    Lan, Jiao
    Jiao, Wei
    Mo, Xianglan
    Huang, Yongta
    Ye, Huilan
    Xiao, Ruiping
    Wang, Yongli
    Mo, Mingzheng
    Shi, Liwei
    ONCOLOGY LETTERS, 2017, 14 (03) : 2669 - 2676
  • [37] Transcriptomic sequencing analysis of key long noncoding RNAs and mRNAs expression profiles in postoperative recurrence of hepatocellular carcinoma
    Yao, Xiaohui
    Liu, Shan
    Xia, Huan
    Li, Hanhan
    Wang, Zhijie
    Su, Le
    Guo, Wei
    Chen, Hanrui
    TECHNOLOGY AND HEALTH CARE, 2024, 32 (02) : 735 - 747
  • [38] Prognostic significance of the long noncoding RNAs in nasopharyngeal carcinoma: a systematic review and meta-analysis
    Guo, HuanHuan
    Huang, Shuo
    Li, Shuang
    Yu, Hui
    Wu, ShiXiong
    Zhou, XuHong
    CANCER MANAGEMENT AND RESEARCH, 2018, 10 : 1763 - 1779
  • [39] Six long noncoding RNAs as potentially biomarkers involved in competitive endogenous RNA of hepatocellular carcinoma
    Qu, Lili
    Cai, Xiaoxiao
    Xu, Junwen
    Wei, Xiaowei
    Qu, Xiaojun
    Sun, Li
    Gong, Lailing
    Su, Chuan
    Zh, Yefei
    CLINICAL AND EXPERIMENTAL MEDICINE, 2020, 20 (03) : 437 - 447
  • [40] Six long noncoding RNAs as potentially biomarkers involved in competitive endogenous RNA of hepatocellular carcinoma
    Lili Qu
    Xiaoxiao Cai
    Junwen Xu
    Xiaowei Wei
    Xiaojun Qu
    Li Sun
    Lailing Gong
    Chuan Su
    Yefei Zhu
    Clinical and Experimental Medicine, 2020, 20 : 437 - 447