New Sea Anemone Toxin RTX-VI Selectively Modulates Voltage-Gated Sodium Channels

被引：3

作者：

Kalina, R. S. ^{[1
]}

Peigneur, S. ^{[2
]}

Gladkikh, I. N. ^{[1
]}

Dmitrenok, P. S. ^{[1
]}

Kim, N. Y. ^{[1
]}

Leychenko, E., V ^{[1
]}

Monastyrnaya, M. M. ^{[1
]}

Tytgat, J. ^{[2
]}

Kozlovskaya, E. P. ^{[1
]}

机构：

[1] Russian Acad Sci, Elyakov Pacific Inst Bioorgan Chem, Far Eastern Branch, Vladivostok, Russia

[2] Univ Leuven, Dept Toxicol & Pharmacol, Leuven, Belgium

来源：

DOKLADY BIOCHEMISTRY AND BIOPHYSICS | 2020年 / 495卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

sea anemone; Heteractis crispa; voltage-gated sodium channels; toxins; electrophysiology; PEPTIDE; SEQUENCE;

D O I：

10.1134/S1607672920060071

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new neurotoxin RTX-VI that modulates the voltage-gated sodium channels (Na-V) was isolated from the ethanolic extract of the sea anemone Heteractis crispa. Its amino acid sequence was determined using the combination of Edman degradation and tandem mass spectrometry. RTX-VI turned out to be an unusual natural analogue of the previously described sea anemone toxin RTX-III. The RTX-VI molecule consists of two disulfide-linked peptide chains and is devoid of Arg13, which is important for the selectivity and affinity of such peptides for the Na-V channels. Electrophysiological screening of RTV-VI on Na-V channel subtypes showed its selective interaction with the central nervous system (Na(V)1.2, Na(V)1.6) and insect (BgNa(V)1, VdNa(V)1) sodium channels.

引用

页码：292 / 295

页数：4

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