Virulence and genotypic characterization of Listeria monocytogenes isolated from vegetable and soil samples

被引:60
作者
Soni, Dharmendra Kumar [1 ]
Singh, Major [2 ]
Singh, Durg Vijai [3 ]
Dubey, Suresh Kumar [1 ]
机构
[1] Banaras Hindu Univ, Dept Bot, Varanasi 221005, Uttar Pradesh, India
[2] Indian Inst Vegetable Res, Varanasi 221305, Uttar Pradesh, India
[3] Inst Life Sci, Bhubaneswar 751023, Orissa, India
关键词
Serotype identification; ERIC- and REP-PCR; Virulence genes; Multiplex PCR; FIELD GEL-ELECTROPHORESIS; PHOSPHOLIPASE-C ACTIVITY; STRAINS; MILK; PCR; IDENTIFICATION; FOOD; EPIDEMIOLOGY; TRANSMISSION; SEQUENCES;
D O I
10.1186/s12866-014-0241-3
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: Listeria monocytogenes, a foodborne pathogen is ubiquitous to different environments including the agroecosystem. The organism poses serious public health problem. Therefore, an attempt has been made to gain further insight to their antibiotic susceptibility, serotypes and the virulence genes. Results: Out of the 10 vegetables selected, 6 (brinjal, cauliflower, dolichos-bean, tomato, chappan-kaddu and chilli), 20 isolates (10%) tested positive for L. monocytogenes. The prevalence of the pathogen in the respective rhizosphere soil samples was 5%. Noticeably, L. monocytogenes was absent from only cabbage, broccoli, palak and cowpea, and also the respective rhizospheric soils. The 30 isolates +ve for pathogenicity, belonged to serogroup 4b, 4d or 4e, and all were positive for inlA, inlC, inlJ, plcA, prfA, actA, hlyA and iap gene except one (VC3) among the vegetable isolates that lacked the plcA gene. ERIC- and REP-PCR collectively revealed that isolates from vegetables and their respective rhizospheric soils had distinct PCR fingerprints. Conclusions: The study demonstrates the prevalence of pathogenic L. monocytogenes in the selected agricultural farm samples. The increase in the number of strains resistant to ciprofloxacin and/or cefoxitin seems to pose serious public health consequences.
引用
收藏
页数:10
相关论文
共 63 条
[1]  
[Anonymous], 1997, 112901 EN ISO
[2]   Genotypic characterization of Listeria monocytogenes isolated from milk and ready-to-eat indigenous milk products [J].
Aurora, Ritu ;
Prakash, Alka ;
Prakash, Sant .
FOOD CONTROL, 2009, 20 (09) :835-839
[3]   Epidemiology and risk management of listeriosis in India [J].
Barbuddhe, S. B. ;
Malik, S. V. S. ;
Kumar, J. Ashok ;
Kalorey, D. R. ;
Chakraborty, T. .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2012, 154 (03) :113-118
[4]  
BAUER AW, 1966, AM J CLIN PATHOL, V45, P493
[5]  
BEUCHAT LR, 1990, T IND MICR, P175
[6]  
[CDC] Centre for Disease Control and Prevention, 2011, INV UPD MULT OUTBR L
[7]   Antibiotic resistance in Listeria spp. [J].
Charpentier, E ;
Courvalin, P .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (09) :2103-2108
[8]   INCIDENCE OF ANTIBIOTIC-RESISTANCE IN LISTERIA SPECIES [J].
CHARPENTIER, E ;
GERBAUD, G ;
JACQUET, C ;
ROCOURT, J ;
COURVALIN, P .
JOURNAL OF INFECTIOUS DISEASES, 1995, 172 (01) :277-281
[9]   Prevalence and contamination patterns of Listeria monocytogenes in catfish processing environment and fresh fillets [J].
Chen, Bang-Yuan ;
Pyla, Rajkumar ;
Kim, Tae-Jo ;
Silva, Juan L. ;
Jung, Yean-Sung .
FOOD MICROBIOLOGY, 2010, 27 (05) :645-652
[10]  
Chukwu OOC, 2006, J MICROBIOL, V20, P900