A Facile Method for Preparation of Tailored Scaffolds for DNA-Origami

被引：39

作者：

Erkelenz, Michael ^{[1
]}

Bauer, Dennis M. ^{[1
,2
]}

Meyer, Rebecca ^{[1
,5
]}

Gatsogiannis, Christos ^{[3
]}

Raunser, Stefan ^{[3
]}

Sacca, Barbara ^{[1
,4
]}

Niemeyer, Christof M. ^{[1
,5
]}

机构：

[1] Tech Univ Dortmund, Fak Chem, D-44227 Dortmund, Germany

[2] Karlsruhe Inst Technol KIT, DFG Ctr Funct Nanostruct CFN, D-76131 Karlsruhe, Germany

[3] Max Planck Inst Mol Physiol, D-44227 Dortmund, Germany

[4] Univ Duisburg Essen, Zentrum Med Biotechnol ZMB, Fak Biol, D-45117 Essen, Germany

[5] Karlsruhe Inst Technol KIT, Inst Biol Interfaces IBG 1, D-76344 Eggenstein Leopoldshafen, Germany

来源：

SMALL | 2014年 / 10卷 / 01期

关键词：

bacterial cloning; DNA; nanostructures; self-assembly; FOLDING DNA; NANOSCALE SHAPES;

D O I：

10.1002/smll.201300701

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A convenient PCR cloning strategy allows one to prepare hundreds of picomoles of circular single-stranded DNA molecules, which are suitable as scaffolds for the assembly of DNA origami structures. The method is based on a combination of site-directed mutagenesis and site- and ligation-independent cloning protocols, with simultaneous insertion of a nicking endonuclease restriction site on a double-stranded plasmid of desired length and sequence. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：73 / 77

页数：5

共 15 条

[1] DNA origami design of dolphin-shaped structures with flexible tails [J].

Andersen, Ebbe S. ;

Dong, Mingdong ;

Nielsen, Morten M. ;

Jahn, Kasper ;

Lind-Thomsen, Allan ;

Mamdouh, Wael ;

Gothelf, Kurt V. ;

Besenbacher, Flemming ;

Kjems, Jorgen .

ACS NANO, 2008, 2 (06) :1213-1218

[2] Folding DNA into Twisted and Curved Nanoscale Shapes [J].

Dietz, Hendrik ;

Douglas, Shawn M. ;

Shih, William M. .

SCIENCE, 2009, 325 (5941) :725-730

[3] Rapid prototyping of 3D DNA-origami shapes with caDNAno [J].

Douglas, Shawn M. ;

Marblestone, Adam H. ;

Teerapittayanon, Surat ;

Vazquez, Alejandro ;

Church, George M. ;

Shih, William M. .

NUCLEIC ACIDS RESEARCH, 2009, 37 (15) :5001-5006

[4] Self-assembly of DNA into nanoscale three-dimensional shapes [J].

Douglas, Shawn M. ;

Dietz, Hendrik ;

Liedl, Tim ;

Hoegberg, Bjoern ;

Graf, Franziska ;

Shih, William M. .

NATURE, 2009, 459 (7245) :414-418

[5] Folding DNA Origami from a Double-Stranded Source of Scaffold [J].

Hogberg, Bjorn ;

Liedl, Tim ;

Shih, William M. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (26) :9154-+

[6] Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC [J].

Li, Mamie Z. ;

Elledge, Stephen J. .

NATURE METHODS, 2007, 4 (03) :251-256

[7] DNA origami: a history and current perspective [J].

Nangreave, Jeanette ;

Han, Dongran ;

Liu, Yan ;

Yan, Hao .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2010, 14 (05) :608-615

[8] Polymerase Chain Reaction Based Scaffold Preparation for the Production of Thin, Branched DNA Origami Nanostructures of Arbitrary Sizes [J].

Pound, Elisabeth ;

Ashton, Jeffrey R. ;

Becerril, Hector A. ;

Woolley, Adam T. .

NANO LETTERS, 2009, 9 (12) :4302-4305

[9] Folding DNA to create nanoscale shapes and patterns [J].

Rothemund, PWK .

NATURE, 2006, 440 (7082) :297-302

[10] DNA Origami: The Art of Folding DNA [J].

Sacca, Barbara ;

Niemeyer, Christof M. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 51 (01) :58-66

← 1 2 →