A Desaturase Gene Involved in the Formation of 1,14-Nonadecadiene in Synechococcus sp Strain PCC 7002

被引：13

作者：

Mendez-Perez, Daniel ^{[1
]}

Herman, Nicolaus A. ^{[1
]}

Pfleger, Brian F. ^{[1
,2
]}

机构：

[1] Univ Wisconsin, Dept Chem & Biol Engn, Madison, WI 53706 USA

[2] Univ Wisconsin, Microbiol Doctoral Training Program, Madison, WI USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2014年 / 80卷 / 19期

关键词：

ACYL-LIPID DESATURASES; BLUE-GREEN-ALGAE; PROTEIN; BIOSYNTHESIS;

D O I：

10.1128/AEM.01615-14

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The marine cyanobacterium Synechococcus sp. strain PCC 7002 synthesizes two alkenes, 1-nonadecene and 1,14-nonadecadiene. Whereas the genetic basis for the biosynthesis of the terminal double bond in both alkenes has been characterized, the origin of the internal double bond in 1,14-nonadecadiene has not. In this study, we demonstrate that a gene encoding an uncharacterized desaturase is involved in the formation of the internal double bond of 1,14-nonadecadiene. Further, at low temperatures, the desaturase gene is essential for growth, and in wild-type cells the levels of 1,14-nonadecadiene increase relative to that of cells grown at 38 degrees C. These data suggest that 1,14-nonadecadiene plays a role in responding to cold stress.

引用

页码：6073 / 6079

页数：7

共 15 条

[1] Modular Synthase-Encoding Gene Involved in α-Olefin Biosynthesis in Synechococcus sp Strain PCC 7002
Mendez-Perez, Daniel
Begemann, Matthew B.
Pfleger, Brian F.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (12) : 4264 - 4267
[2] Production of γ-linolenic acid and stearidonic acid by Synechococcus sp PCC7002 containing cyanobacterial fatty acid desaturase genes
Dong Xuewei
He Qingfang
Peng Zhenying
Yu Jinhui
Bian Fei
Li Youzhi
Bi Yuping
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY, 2016, 34 (04): : 772 - 780
[3] Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp strain PCC 7002: Cell factories for soluble sugars
Xu, Yu
Guerra, L. Tiago
Li, Zhongkui
Ludwig, Marcus
Dismukes, G. Charles
Bryant, Donald A.
METABOLIC ENGINEERING, 2013, 16 : 56 - 67
[4] Distinct and redundant functions of three homologs of RNase III in the cyanobacterium Synechococcus sp strain PCC 7002
Gordon, Gina C.
Cameron, Jeffrey C.
Pfleger, Brian F.
NUCLEIC ACIDS RESEARCH, 2018, 46 (04) : 1984 - 1997
[5] Complementation of Cobalamin Auxotrophy in Synechococcus sp Strain PCC 7002 and Validation of a Putative Cobalamin Riboswitch In Vivo
Perez, Adam A.
Liu, Zhenfeng
Rodionov, Dmitry A.
Li, Zhongkui
Bryant, Donald A.
JOURNAL OF BACTERIOLOGY, 2016, 198 (19) : 2743 - 2752
[6] The proteins involved in sucrose synthesis in the marine cyanobacterium Synechococcus sp. PCC 7002 are encoded by two genes transcribed from a gene cluster
Cumino, Andrea C.
Perez-Cenci, Macarena
Giarrocco, Laura E.
Salerno, Graciela L.
FEBS LETTERS, 2010, 584 (22) : 4655 - 4660
[7] Vipp1 Is Essential for the Biogenesis of Photosystem I but Not Thylakoid Membranes in Synechococcus sp PCC 7002
Zhang, Shuyi
Shen, Gaozhong
Li, Zhongkui
Golbeck, John H.
Bryant, Donald A.
JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (23) : 15904 - 15914
[8] Diversity and Evolution of Iron Uptake Pathways in Marine Cyanobacteria from the Perspective of the Coastal Strain Synechococcus sp. Strain PCC 7002
Yong, Cheng-Wen
Deng, Bin
Liu, Ling-Mei
Wang, Xin-Wei
Jiang, Hai-Bo
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2023, 89 (01)
[9] Zn2+- Inducible Expression Platform for Synechococcus sp Strain PCC 7002 Based on the smtA Promoter/Operator and smtB Repressor
Perez, Adam A.
Gajewski, John P.
Ferlez, Bryan H.
Ludwig, Marcus
Baker, Carol S.
Golbeck, John H.
Bryant, Donald A.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2017, 83 (03)
[10] Engineering of Synechococcus sp. strain PCC 7002 for the photoautotrophic production of light-sensitive riboflavin (vitamin B2)
Kachel, Benjamin
Mack, Matthias
METABOLIC ENGINEERING, 2020, 62 : 275 - 286

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