A review of enhancers for chemiluminescence enzyme immunoassay

被引:50
作者
Chen, Ge [1 ]
Jin, Maojun [1 ]
Du, Pengfei [1 ]
Zhang, Chan [1 ]
Cui, Xueyan [1 ]
Zhang, Yudan [1 ]
Wang, Jing [1 ]
Jin, Fen [1 ]
She, Yongxin [1 ]
Shao, Hua [1 ]
Wang, Shanshan [1 ]
Zheng, Lufei [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Key Lab Agroprod Qual & Food Safety, Beijing 100081, Peoples R China
关键词
Chemiluminescence immunoassay; enhancer; HRP-luminol-H2O2; system; ALP-AMPPD system; PEROXIDASE-INDUCED CHEMILUMINESCENCE; HORSERADISH-PEROXIDASE; LUMINOL; SIGNAL; LUMINESCENCE; MECHANISM; MILK; DERIVATIVES; EMPLOYMENT; SAMPLES;
D O I
10.1080/09540105.2016.1272550
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
There is increasing interest for chemiluminescence (CL) detection with the characteristics of simplicity, low cost and high sensitivity, especially wide application of enhancersin CL detection to increased signals, prolonged luminescence time and enhanced intensity. In this review, the applications of primary enhancers and secondary enhancers were mainly described in the horseradish peroxidase-luminol system of CL enzyme immunoassay for light delay in the course of the reaction with improved sensitivity. The present review on enhancers covers the papers since 1983. Future research needs to develop novel enhancers with less interference and better performance. With wide utilization of enhancers in the CL system, the CL immunodetection technology showed a good potential and wide development prospects in food and medicine fields.
引用
收藏
页码:315 / 327
页数:13
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