Role of placenta-specific protein 1 in trophoblast invasion and migration

被引：42

作者：

Chang, Wen-Lin ^{[1
,2
]}

Yang, Qing ^{[1
]}

Zhang, Hui

Lin, Hai-Yan ^{[2
,3
]}

Zhou, Zhi ^{[2
,4
]}

Lu, Xiaoyin ^{[2
,4
]}

Zhu, Cheng ^{[2
]}

Xue, Li-Qun ^{[1
]}

Wang, Hongmei ^{[2
]}

机构：

[1] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China

[2] Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China

[3] Capital Med Univ, Beijing Obstet & Gynecol Hosp, Beijing 100069, Peoples R China

[4] Grad Sch Chinese Acad Sci, Beijing 100039, Peoples R China

来源：

REPRODUCTION | 2014年 / 148卷 / 04期

关键词：

VILLOUS TREE; FETAL-GROWTH; PLAC1; EXPRESSION; CELL; DIFFERENTIATION; GENE; PREGNANCY; SYNCYTIOTROPHOBLAST; PREECLAMPSIA;

D O I：

10.1530/REP-14-0052

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Placenta-specific protein 1 (PLAC1), a placenta-specific gene, is known to be involved in the development of placenta in both humans and mice. However, the precise role of PLAC1 in placental trophoblast function remains unclear. In this study, the localization of PLAC1 in human placental tissues and its physiological significance in trophoblast invasion and migration are investigated by technical studies including real-time RT-PCR, in situ hybridization, immunohistochemistry, and functional studies by utilizing cell invasion and migration assays in the trophoblast cell line HTR8/SVneo as well as the primary inducing extravillous trophoblasts (EVTs). The results show that PLAC1 is mainly detected in the trophoblast columns and syncytiotrophoblast of the first-trimester human placental villi, as well as in the EVTs that invade into the maternal decidua. Knockdown of PLAC1 by RNA interference significantly suppresses the invasion and migration of HTR8/SVneo cells and shortens the distance of the outgrowth of the induced EVTs from the cytotrophoblast column of the explants. All the above data suggests that PLAC1 plays an important role in human placental trophoblast invasion and migration.

引用

页码：343 / 352

页数：10

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