Differential expression of integrin subunits in canine knee ligament fibroblasts

被引:12
作者
Bhargava, MM
Beavis, AJ
Edberg, JC
Warren, RF
Attia, ET
Hannafin, JA
机构
[1] Cornell Univ, Med Ctr, New York Hosp, Hosp Special Surg,Lab Soft Tissue Res, New York, NY 10021 USA
[2] Cornell Univ, Med Ctr, New York Hosp, Hosp Special Surg,Flow Cytometry Core Facil, New York, NY 10021 USA
关键词
D O I
10.1002/jor.1100170520
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
A method for measuring the expression of integrin subunits on the cell surface of knee ligament fibroblasts was developed with use of flow cytometry and immunofluorescence. The ligament cells exhibited uniform size and density, as shown by forward and side-scatter properties, and showed minimal nonspecific binding of isotype control antibodies compared with unstained cells. All cells expressed the alpha 5 integrin subunit; lateral collateral ligament cells stained with antibody to alpha 5 showed a mean fluorescence intensity 2-fold higher than that of medial collateral ligament cells, 1.5-fold higher than that of posterior cruciate ligament cells, and 3-fold higher than that of anterior cruciate ligament cells, indicating a greater expression of the alpha 5 subunit by lateral collateral ligament cells than by medial collateral, posterior cruciate, and anterior cruciate ligament cells. All cells expressed the pi integrin subunit; the expression by posterior cruciate ligament cells was 3-fold higher than that by medial collateral ligament or lateral collateral ligament cells and 5-fold higher than that by anterior cruciate ligament cells. All cells expressed the beta 3 integrin subunit; the expression by posterior cruciate ligament cells was 1.5, 3, and 4.5-fold greater than that by lateral collateral, anterior cruciate, and medial collateral ligament cells, respectively. Our data suggest there is a differential expression of integrin subunits in knee ligament fibroblasts, and this in part may explain differences in their attachment and adherence to extracellular matrix molecules.
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页码:748 / 754
页数:7
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