Pseudomonas aeruginosa flagellin as an adjuvant: superiority of a conjugated form of flagellin versus a mixture with a human immunodeficiency virus type 1 vaccine candidate in the induction of immune responses

被引：16

作者：

Delavari, Safura ^{[1
]}

Sohrabi, Mojtaba ^{[1
]}

Ardestani, Mehdi Shafiee ^{[2
]}

Faezi, Sobhan ^{[3
]}

Tebianian, Majid ^{[4
]}

Taghizadeh, Morteza ^{[5
]}

Shajiei, Arezoo ^{[6
]}

Hosseini, Seyed Younes ^{[7
]}

Moghaddampour, Masoud ^{[5
,8
]}

Mahdavi, Mehdi ^{[9
]}

机构：

[1] Islamic Azad Univ, Qom Branch, Fac Sci, Dept Biol, Qom, Iran

[2] Univ Tehran Med Sci, Fac Pharm, Dept Radiopharm, Tehran, Iran

[3] Pasteur Inst Iran, Dept Mycobacteriol & Pulm Res, Tehran, Iran

[4] Razi Vaccine & Serum Res Inst, Dept Biotechnol, Karaj, Iran

[5] Razi Vaccine & Serum Res Inst, Dept Med Virol, Karaj, Iran

[6] Mashhad Univ Med Sci, Ghaem Hosp, Canc Mol Pathol Res Ctr, Mol Pathol Lab, Mashhad, Iran

[7] Shiraz Univ Med Sci, Dept Bacteriol & Virol, Shiraz, Iran

[8] Iran Univ Med Sci, Sch Med, Dept Virol, Tehran, Iran

[9] Pasteur Inst Iran, Dept Immunol, Tehran, Iran

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 2015年 / 64卷

关键词：

D O I：

10.1099/jmm.0.000174

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In the present study, the adjuvant activity of flagellin was compared, in the conjugated and mixed forms, against a peptide vaccine from human immunodeficiency virus type 1 (HIV-1) p24-Nef vaccine candidate. Mice were immunized with the HIV-1 p24-Nef peptide with flagellin in both conjugated and mixed forms. Lymphocyte proliferation, CTL activity, and IL-4 and IFN-gamma cytokines were evaluated by bromodeoxyuridine, carboxyfluorescein succinimidyl ester and ELISA methods, respectively. At the same time, the frequency of IFN-gamma-producing T-lymphocytes, as well as total and isotype-specific antibodies, were assessed by ELISPOT and indirect ELISA, respectively. Our experimental results showed that the immunized mice with the HIV-1 p24-Nef conjugated or mixed forms of flagellin led to increases in the proliferative responses and Th1 cytokine pattern. The conjugated form of vaccine led to increased CTL activity and a Th1 cytokine pattern of immune responses, as well as an IgM isotype of humoral responses in comparison with the mixed form. The flagellin-conjugated vaccine seems to be more potent in increasing vaccine immunogenicity.

引用

页码：1361 / 1368

页数：8

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