A ribosome-inactivating protein from Amaranthus viridis

被引：26

作者：

Kwon, SY

An, CS

Liu, JR

Paek, KH

机构：

[1] KOREA RES INST BIOSCI & BIOTECHNOL, TAEJON 305333, SOUTH KOREA

[2] SEOUL NATL UNIV, DEPT BIOL, SEOUL 151742, SOUTH KOREA

[3] KOREA UNIV, GRAD SCH BIOTECHNOL, SEOUL 136701, SOUTH KOREA

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 09期

关键词：

antiviral protein; Amaranthus viridis; in vitro translation; RIP; TMV;

D O I：

10.1271/bbb.61.1613

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An antiviral protein purified from the leaves of Amaranthus viridis was named amaranthin. The in vivo antiviral activity of amaranthin was confirmed in tobacco mosaic virus (TMV) infection test on Nicotiana glutinosa leaves, The molecular mass of the amaranthin was estimated about 30 kDa by SDS-PAGE and the pI was measured as 9.8 by isoelectric focusing (IEF) analysis, Cytotoxicity of the amaranthin using in vitro translation inhibition assay was similar to that of pokeweed antiviral protein (PAP) with IC50 of 25 pM. Depurination activity (N-glycosidase activity) against animal rRNA was also confirmed.

引用

页码：1613 / 1614

页数：2

共 50 条

[31] Metabolites of the Vietnamese Plant Amaranthus viridis [J].

Cao Thi Hue ;

Nguyen Thi Tu Oanh ;

Tran Huu Giap ;

Nguyen Thi Minh Hang ;

N. P. Mishchenko ;

S. A. Fedoreev ;

E. V. Spiridovich ;

Chau Van Minh ;

Nguyen Quoc Vuong ;

Le Nguyen Thanh .

Chemistry of Natural Compounds, 2017, 53 :1150-1151

[32] Structure-activity relationships of saponins enhancing the cytotoxicity of ribosome-inactivating proteins type I (RIP-I) [J].

Boettger, Stefan ;

Westhof, Elena ;

Siems, Karsten ;

Melzig, Matthias F. .

TOXICON, 2013, 73 :144-150

[33] Mechanism of Anti-HIV Activity of Ribosome Inactivating Protein, Saporin [J].

Yadav, Santosh K. ;

Batra, Janendra K. .

PROTEIN AND PEPTIDE LETTERS, 2015, 22 (06) :497-503

[34] The interaction of shikimic acid and protein phosphorylation with PEP carboxylase from the C4 dicot Amaranthus viridis [J].

Colombo, SL ;

Andreo, CS ;

Chollet, R .

PHYTOCHEMISTRY, 1998, 48 (01) :55-59

[35] A novel antiproliferative and antifungal lectin from Amaranthus viridis Linn seeds [J].

Kaur, Navjot ;

Dhuna, Vikram ;

Kamboj, Sukhdev Singh ;

Agrewala, Javed N. ;

Singh, Jatinder .

PROTEIN AND PEPTIDE LETTERS, 2006, 13 (09) :895-905

[36] Purification and Characterization of a New Ribosome Inactivating Protein from Cinchonaglycoside C-treated Tobacco Leaves [J].

Yanmei LiYantao JiaZhongkai ZhangXiaoying ChenHongping HeRongxiang Fang and Xiaojiang Hao State Key Laboratory of Photochemistry and Plant Resources in West ChinaKunming Institute of Botanythe Chinese Academy of SciencesKunming China ;