A ribosome-inactivating protein from Amaranthus viridis

被引：26
|
作者：
Kwon, SY
An, CS
Liu, JR
Paek, KH
机构：
[1] KOREA RES INST BIOSCI & BIOTECHNOL, TAEJON 305333, SOUTH KOREA
[2] SEOUL NATL UNIV, DEPT BIOL, SEOUL 151742, SOUTH KOREA
[3] KOREA UNIV, GRAD SCH BIOTECHNOL, SEOUL 136701, SOUTH KOREA
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 09期
关键词：
antiviral protein; Amaranthus viridis; in vitro translation; RIP; TMV;
D O I：
10.1271/bbb.61.1613
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An antiviral protein purified from the leaves of Amaranthus viridis was named amaranthin. The in vivo antiviral activity of amaranthin was confirmed in tobacco mosaic virus (TMV) infection test on Nicotiana glutinosa leaves, The molecular mass of the amaranthin was estimated about 30 kDa by SDS-PAGE and the pI was measured as 9.8 by isoelectric focusing (IEF) analysis, Cytotoxicity of the amaranthin using in vitro translation inhibition assay was similar to that of pokeweed antiviral protein (PAP) with IC50 of 25 pM. Depurination activity (N-glycosidase activity) against animal rRNA was also confirmed.
引用
收藏
页码：1613 / 1614
页数：2
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