Best Practices for the Use of Micropipets

被引：0

作者：

Carle, A. Bjoern ^{[1
]}

Rumery, Doreen ^{[1
]}

Rodrigues, George ^{[1
]}

机构：

[1] Artel, Westbrook, ME 04092 USA

来源：

AMERICAN LABORATORY | 2014年 / 46卷 / 05期

关键词：

Gases; -; Pistons;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Experts share their views on some of the best practices that need to be adopted for the use of micropipets. Piston-operated air-displacement pipets use an air cushion to couple the pipet's piston to the aspirated liquid inside of the pipet tip. The Ideal Gas Law allows one to estimate the effects that temperature, evaporation, and the ratio of captive air volume to the pipet's set volume will have on the aspirated and delivered volume of a pipetting cycle. It is recommended that the pipet, the pipet tip, and the sample solution have been equilibrated for at least 2 hours and are within 0.5 °C of ambient temperature for accurate pipet results. Many samples need to be handled at specific high or low temperatures, and pipetting such samples can introduce significant errors in the delivered volume due to the expansion or contraction of the captive air volume and evaporation.

引用

页码：14 / 17

页数：4