Real-time monitoring of rolling circle amplification using aggregation-induced emission: applications in biological detection

被引：39

作者：

Jiang, Hong-Xin ^{[1
,2
,3
]}

Zhao, Meng-Yao ^{[1
,2
,3
]}

Niu, Chen-Di ^{[1
,2
,3
]}

Kong, De-Ming ^{[1
,2
,3
]}

机构：

[1] Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China

[2] Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China

[3] Nankai Univ, Tianjin Key Lab Biosensing & Mol Recognit, Res Ctr Analyt Sci, Tianjin 300071, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 92期

基金：

中国国家自然科学基金;

关键词：

FLUORESCENT-PROBE; DNA-LIGASE; MOLECULAR BEACONS; PROTEIN; QUANTIFICATION; APOPTOSIS; DNAZYME; ASSAY;

D O I：

10.1039/c5cc07340e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report that QATPE, an aggregation-induced emission-active tetraphenylethene dye, can be used as a non-sequence-specific ssDNA probe for real-time monitoring of all rolling circle amplification (RCA) reactions, thus making RCA more suitable for biosensing applications.

引用

页码：16518 / 16521

页数：4

共 31 条

[1] Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine [J].

Ali, M. Monsur ;

Li, Feng ;

Zhang, Zhiqing ;

Zhang, Kaixiang ;

Kang, Dong-Ku ;

Ankrum, James A. ;

Le, X. Chris ;

Zhao, Weian .

CHEMICAL SOCIETY REVIEWS, 2014, 43 (10) :3324-3341

[2]

Aray M., 2005, EXPERT REV MOL DIAGN, V5, P209

[3] Development of a SYBR Green real-time PCR assay with melting curve analysis for simultaneous detection and differentiation of canine adenovirus type 1 and type 2 [J].

Balboni, Andrea ;

Dondi, Francesco ;

Prosperi, Santino ;

Battilani, Mara .

JOURNAL OF VIROLOGICAL METHODS, 2015, 222 :34-40

[4] Periodic DNA nanotemplates synthesized by rolling circle amplification [J].

Beyer, S ;

Nickels, P ;

Simmel, FC .

NANO LETTERS, 2005, 5 (04) :719-722

[5] Sensitive and Label-Free DNA Methylation Detection by Ligation-Mediated Hyperbranched Rolling Circle Amplification [J].

Cao, Anping ;

Zhang, Chun-yang .

ANALYTICAL CHEMISTRY, 2012, 84 (14) :6199-6205

[6]

Cheglakov Z., 2008, ANGEW CHEM, V120, P132

[7] Nanostructures - Increasing the complexity of periodic protein nanostructures by the rolling-circle-amplified synthesis of aptamers [J].

Cheglakov, Zoya ;

Weizmann, Yossi ;

Braunschweig, Adam B. ;

Wilner, Ofer L. ;

Willner, Itamar .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (01) :126-130

[8] Glucosamine hydrochloride functionalized tetraphenylethylene: A novel fluorescent probe for alkaline phosphatase based on the aggregation-induced emission [J].

Chen, Qi ;

Bian, Ning ;

Cao, Chun ;

Qiu, Xi-Long ;

Qi, Ai-Di ;

Han, Bao-Hang .

CHEMICAL COMMUNICATIONS, 2010, 46 (23) :4067-4069

[9] Enzyme-Regulated Activation of DNAzyme: A Novel Strategy for a Label-Free Colorimetric DNA Ligase Assay and Ligase-Based Biosensing [J].

He, Kaiyu ;

Li, Wang ;

Nie, Zhou ;

Huang, Yan ;

Liu, Zhuoliang ;

Nie, Lihua ;

Yao, Shouzhuo .

CHEMISTRY-A EUROPEAN JOURNAL, 2012, 18 (13) :3992-3999

[10] Label-free fluorescent probing of G-quadruplex formation and real-time monitoring of DNA folding by a quaternized tetraphenylethene salt with aggregation-induced emission characteristics [J].

Hong, Yuning ;

Haeussler, Matthias ;

Lam, Jacky W. Y. ;

Li, Zhen ;

Sin, King Keung ;

Dong, Yongqiang ;

Tong, Hui ;

Liu, Jianzhao ;

Qin, Anjun ;

Renneberg, Reinhard ;

Tang, Ben Zhong .

CHEMISTRY-A EUROPEAN JOURNAL, 2008, 14 (21) :6428-6437

← 1 2 3 4 →