Rapid homogeneous immunoassay of peptides based on bioluminescence resonance energy transfer from firefly luciferase

被引：10

作者：

Yamakawa, Y ^{[1
]}

Ueda, H ^{[1
]}

Kitayama, A ^{[1
]}

Nagamune, T ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2002年 / 93卷 / 06期

关键词：

fusion protein; bioluminescent protein; homogeneous immunoassay; peptide epitope;

D O I：

10.1263/jbb.93.537

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A sensitive homogeneous immunoassay is needed in the field of clinical diagnostics. Here we propose a rapid and potentially sensitive homogeneous immunoassay of peptide epitope based on the bioluminescence resonance energy transfer (BRET). A GST-peptide tag fused to the N-terminus of firefly luciferase, and Cy3 or Cy3.5-labeled anti-peptide antibody were prepared to detect BRET from the hybrid luciferase to the fluorolabeled antibody. By measuring the spectral change due to BRET, the amount of c-myc peptide was successfully determined in a short period.

引用

页码：537 / 542

页数：6

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