Screening of Xanthine Oxidase Inhibitors in Traditional Chinese Herbal Formulae Ermiaowan Using Ultrafiltration UPLC-MSn

被引：7

作者：

Xu Chen ^{[1
,2
]}

Liu Shu ^{[1
]}

Liu Zhiqiang ^{[1
]}

Song Fengrui ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, Natl Ctr Mass Spectrometry Changchun, Jilin Prov Key Lab Tradit Chinese Med Chem & Mass, Changchun 130022, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2014年 / 35卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Ermiaowan; Xanthine oxidase; Ultrafiltration UPLC/Q-TOF/MS; ALPHA-GLUCOSIDASE INHIBITORS; MASS-SPECTROMETRY; FREE-RADICALS; URIC-ACID; EXTRACT; SERUM; ANTIOXIDANTS; IONIZATION; ALKALOIDS; ASSAY;

D O I：

10.7503/cjcu20140419

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An ultrafiltration UPLC/Q-TOF/MS assay and in vitro xanthine oxidase inhibition assay were combined to screen xanthine oxidase inhibitors in traditional Chinese herbal formulae Ermiaowan. The result of in vitro xanthine oxidase inhibition assay showed that Ermiaowan had a strong xanthine oxidase inhibitory activity, the IC50 value was (0.218 +/- 0.0034) mg/mL. Nine compounds were screened and identified as potential xanthine oxidase inhibitors in Ermiaowan using ultrafiltration UPLC/Q-TOF/MS. This work provides a certain basis for the development of xanthine oxidase inhibitors and the mechanism clarification of Ermiaowan to treat gout and hyperuricemia.

引用

页码：1640 / 1645

页数：6

共 20 条

[11] Screening and determination of potential xanthine oxidase inhibitors from Radix Salviae Miltiorrhizae using ultrafiltration liquid chromatography-mass spectrometry [J].

Liu, Yang ;

Liu, Shu ;

Liu, Zhiqiang .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2013, 923 :48-53

[12] UPLC-MS/MS Method for Determination of Uric Acid and Creatinine in Serum and Urine of Hyperuricemic Mice [J].

Ma Qing-Shan ;

Wang Qian ;

Zhao Kai-Shu ;

Zhai Shu-Bo ;

Liu Shu ;

Liu Zhi-Qiang .

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 2013, 34 (12) :2716-2720

[13] Chemical profiling of Wu-tou decoction by UPLC-Q-TOF-MS [J].

Qi, Yao ;

Li, Shizhe ;

Pi, Zifeng ;

Song, Fengrui ;

Lin, Na ;

Liu, Shu ;

Liu, Zhiqiang .

TALANTA, 2014, 118 :21-29

[14] Free radicals and antioxidants in normal physiological functions and human disease [J].

Valko, Marian ;

Leibfritz, Dieter ;

Moncol, Jan ;

Cronin, Mark T. D. ;

Mazur, Milan ;

Telser, Joshua .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2007, 39 (01) :44-84

[15] Pulsed ultrafiltration mass spectrometry: A new method for screening combinatorial libraries [J].

vanBreemen, RB ;

Huang, CR ;

Nikolic, D ;

Woodbury, CP ;

Zhao, YZ ;

Venton, DL .

ANALYTICAL CHEMISTRY, 1997, 69 (11) :2159-2164

[16] Superoxide generated by pyrogallol reduces highly water-soluble tetrazolium salt to produce a soluble formazan: A simple assay for measuring superoxide anion radical scavenging activities of biological and abiological samples [J].

Xu, Chen ;

Liu, Shu ;

Liu, Zhiqiang ;

Song, Fengrui ;

Liu, Shuying .

ANALYTICA CHIMICA ACTA, 2013, 793 :53-60

[17] Monitoring Enzyme Reaction and Screening of Inhibitors of Acetylcholinesterase by Quantitative Matrix-Assisted Laser Desorption/Ionization Fourier Transform Mass Spectrometry [J].

Xu, Zhe ;

Yao, Shengjun ;

Wei, Yuanlong ;

Zhou, Jing ;

Zhang, Li ;

Wang, Cuihong ;

Guo, Yinlong .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2008, 19 (12) :1849-1855

[18] Characterization of isoquinoline alkaloids, diterpenoids and steroids in the Chinese herb Jin-Guo-Lan (Tinospora sagittata and Tinospora capillipes) by high-performance liquid chromatography/electrospray ionization with multistage mass spectrometry [J].

Zhang, Yufeng ;

Shi, Qirong ;

Shi, Peiying ;

Zhang, Weidong ;

Cheng, Yiyu .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2006, 20 (15) :2328-2342

[19] SCREENING FOR α-GLUCOSIDASE INHIBITORS FROM COPTIDIS-RIEHMANNIAE HERB COUPLE BY USING ULTRAFILTRATION LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY [J].

Zhou, Hui ;

Li, Huilin ;

Zheng, Zhong ;

Song, Fengrui ;

Xing, Junpeng ;

Liu, Zhiqiang ;

Liu, Shuying .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2012, 35 (1-4) :1-14

[20] Screening and Structures Characterization of Human Serum Albumin Ligands from Radix Aconiti Alkaloids Extract by Ultrafiltration LC-MSn [J].

Zhu Hong-Bin ;

Liu Shu ;

Wang Chun-Yan ;

Liu Zhi-Qiang ;

Song Feng-Rui .

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 2013, 34 (07) :1635-1639

← 1 2 →