On the preservation of non-covalent protein complexes during electrospray ionization

被引：6
作者：
Chingin, Konstantin [1 ]
Barylyuk, Konstantin [2 ]
Chen, Huanwen [1 ]
机构：
[1] East China Univ Technol, Jiangxi Key Lab Mass Spectrometry & Instrumentat, Guanglan Rd 418, Nanchang 330013, Jiangxi, Peoples R China
[2] Univ Cambridge, Dept Biochem, Hopkins Bldg,Tennis Court Rd, Cambridge CB2 1QW, England
来源：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES | 2016年 / 374卷 / 2079期
基金：
中国国家自然科学基金;
关键词：
native mass spectrometry; thermodynamic equilibrium; accelerated reactions; microdroplet fusion; desorption electrospray ionization; extractive electrospray ionization; MASS-SPECTROMETRY; LIGAND INTERACTIONS; BINDING CONSTANTS; ESI-MS; MECHANISM; DYNAMICS;
D O I：
10.1098/rsta.2015.0377
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The application range of electrospray ionization mass spectrometry for the quantitative determination of stoichiometries and binding constants for non-covalent protein complexes is broadly discussed. The underlying fundamental question is whether or not the original molecular equilibrium can be preserved during the ionization process and be revealed by subsequent mass spectrometry analysis. Here, we take a new look at this question by discussing recent studies in droplet chemistry. This article is part of the themed issue 'Quantitative mass spectrometry'.
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页数：7
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