Piezo1 in Smooth Muscle Cells Is Involved in Hypertension-Dependent Arterial Remodeling

被引:295
作者
Retailleau, Kevin [1 ]
Duprat, Fabrice [1 ]
Arhatte, Malika [1 ]
Ranade, Sanjeev Sumant [2 ]
Peyronnet, Remi [1 ]
Martins, Joana Raquel [1 ]
Jodar, Martine [1 ]
Moro, Celine [1 ]
Offermanns, Stefan [3 ]
Feng, Yuanyi [4 ]
Demolombe, Sophie [1 ]
Patel, Amanda [1 ]
Honore, Eric [1 ]
机构
[1] Univ Nice Sophia Antipolis, Inst Pharmacol Mol & Cellulaire, LabEx ICST, UMR CNRS 7275, F-06560 Valbonne, France
[2] Scripps Res Inst, Howard Hughes Med Inst, Dept Mol & Cellular Neurosci, La Jolla, CA 92037 USA
[3] Max Planck Inst Heart & Lung Res, D-61231 Bad Nauheim, Germany
[4] Northwestern Univ, Dept Neurol, Chicago, IL 60611 USA
关键词
MECHANOSENSITIVE CATION CHANNELS; FILAMIN-A; PERIVENTRICULAR HETEROTOPIA; MYOGENIC CONSTRICTION; SIGNALING MECHANISMS; VASCULAR DEVELOPMENT; TRANSGLUTAMINASE; ION CHANNELS; MUTATIONS; PRESSURE;
D O I
10.1016/j.celrep.2015.09.072
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The mechanically activated non-selective cation channel Piezo1 is a determinant of vascular architecture during early development. Piezo1-deficient embryos die at midgestation with disorganized blood vessels. However, the role of stretch-activated ion channels (SACs) in arterial smooth muscle cells in the adult remains unknown. Here, we show that Piezo1 is highly expressed in myocytes of small-diameter arteries and that smooth-muscle-specific Piezo1 deletion fully impairs SAC activity. While Piezo1 is dispensable for the arterial myogenic tone, it is involved in the structural remodeling of small arteries. Increased Piezo1 opening has a trophic effect on resistance arteries, influencing both diameter and wall thickness in hypertension. Piezo1 mediates a rise in cytosolic calcium and stimulates activity of transglutaminases, cross-linking enzymes required for the remodeling of small arteries. In conclusion, we have established the connection between an early mechanosensitive process, involving Piezo1 in smooth muscle cells, and a clinically relevant arterial remodeling. Piezo1 is a stretch-activated ion channel expressed in the endothelium and its deletion alters vascular architecture. However, its role in arterial myocytes remains unknown. Retailleau et al. show that smooth muscle Piezo1 is dispensable for the arterial myogenic tone but involved in the remodeling of small arteries upon hypertension. © 2015 The Authors.
引用
收藏
页码:1161 / 1171
页数:11
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