Phytochemical Analysis by HPLC-HRESI-MS and Anti-Inflammatory Activity of Tabernaemontana catharinensis

被引：13
作者：
Marques, Jose Ivan [1 ]
Ferreira Alves, Jovelina Samara [1 ]
Torres-Rego, Manoela [2 ]
Furtado, Allanny Alves [2 ]
da Silva Siqueira, Emerson Michell [1 ]
Galinari, Eder [1 ]
de Souza Araujo, Daline Fernandes [3 ]
Bernardo Guerra, Gerlane Coelho [4 ]
de Azevedo, Eduardo Pereira [5 ]
Fernandes-Pedrosa, Matheus de Freitas [2 ]
Zucolotto, Silvana Maria [1 ]
机构：
[1] Univ Fed Rio Grande do Norte, Dept Pharmaceut Sci, Lab Pharmacognosy PNBio, Fac Pharm, Ave Gen Gustavo Cordeiro de Farias S-N, BR-59012570 Natal, RN, Brazil
[2] Univ Fed Rio Grande do Norte, Dept Pharmaceut Sci, Lab Technol & Pharmaceut Biotechnol Tecbiofar, Fac Pharm, Ave Gen Gustavo Cordeiro de Farias S-N, BR-59012570 Natal, RN, Brazil
[3] Univ Fed Rio Grande do Norte, Fac Hlth Sci Trairi, Ave Trairi S-N, BR-59200000 Centro, Santa Cruz, Brazil
[4] Univ Fed Rio Grande do Norte, Biosci Ctr, Dept Biophys & Pharmacol, Campus Univ,Ave Senador Salgado Filho 3000, BR-59072970 Natal, RN, Brazil
[5] Univ Potiguar UnP, Laureate Univ, Grad Program Biotechnol, Ave Senador Salgado Filho 1610, BR-59056000 Natal, RN, Brazil
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2018年 / 19卷 / 02期
关键词：
Apocynaceae; flavonoids; herbal drug; carrageenan; zymosan; AIR-POUCH INFLAMMATION; INDOLE ALKALOIDS; PAW EDEMA; NATURAL-PRODUCTS; FLAVONOIDS; AGENTS; CELLS; COX-2; ISORHAMNETIN; MACROPHAGES;
D O I：
10.3390/ijms19020636
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Tabernaemontana catharinensis (Apocynaceae) has been popularly used by folk medicine because of its anti-inflammatory, analgesic, and antiophidic properties. This study aims to analyze the flavonoids composition of the hydroethanolic extract and of the ethyl acetate (EtOAc) and butanol (BuOH) fractions of T. catharinensis leaves, as well as to evaluate their anti-inflammatory activity using in vivo models. The phytochemical profile, determined by High-Performance Liquid Chromatography-High-Resolution Electrospray Ionization-Mass Spectrometry (HPLC-HRESI-MS), showed the presence of flavonoids mainly having an isorhamnetin nucleus. The anti-inflammatory activity was evaluated in carrageenan-induced paw edema (pre- and post-treatment) with oral administration of a T. catharinensis hydroethanolic extract (50, 100, and 150 mg/kg) and of organic fractions (50 mg/kg). The extract and fractions showed antiedematogenic activity by decreasing myeloperoxidase (MPO) production. In the zymosan-air-pouch model, the extract and fractions inhibited leukocyte migration and significantly decreased the levels of various proteins, such as MPO, interleukin (IL)-1, and tumor necrosis factor (TNF)-. The cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, which revealed no cytotoxicity of the extract and the fractions. These results suggest that the hydroethanolic extract and organic fractions of T. catharinensis leaves have sufficient anti-inflammatory activity to support the popular use of this plant in the treatment of inflammatory disorders.
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页数：19
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