Laser-Fabricated 2D Molybdenum Disulfide Electronic Sensor Arrays for Rapid, Low-Cost, Ultrasensitive Detection of Influenza A and SARS-Cov-2

被引:10
作者
Muratore, Christopher [1 ,2 ]
Muratore, Melani K. [2 ,3 ]
Austin, Drake R. [4 ,5 ]
Miesle, Paige [4 ,5 ,6 ]
Benton, Anna K. [1 ,4 ,5 ]
Beagle, Lucas K. [1 ,4 ,5 ]
Motala, Michael J. [4 ,5 ]
Moore, David C. [4 ,5 ]
Slocik, Joseph M. [4 ,5 ]
Brothers, Michael C. [4 ,7 ]
Kim, Steve S. [7 ]
Krupa, Kristen [1 ]
Back, Tyson A. [5 ]
Grant, John T.
Glavin, Nicholas R. [5 ]
机构
[1] Univ Dayton, Dept Chem & Mat Engn, Dayton, OH 45469 USA
[2] M Nanotech Ltd, Dayton, OH 45409 USA
[3] Univ Dayton, Dept Biol, Dayton, OH 45469 USA
[4] UES Inc, Dayton, OH 45432 USA
[5] Air Force Res Lab, Mat & Mfg Directorate, Dayton, OH 45433 USA
[6] Dept Mech Engn, Dayton, OH 45469 USA
[7] Air Force Res Lab, Human Performance Wing 711, Dayton, OH 45433 USA
关键词
2D materials; antigen test; coronavirus; COVID-19; electronic sensors; MoS; (2); SARS-Cov-2; FIELD-EFFECT TRANSISTOR; GRAPHENE;
D O I
10.1002/admi.202102209
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Multiplex electronic antigen sensors for detection of SARS-Cov-2 spike glycoproteins and hemagglutinin from influenza A are fabricated using scalable processes for straightforward transition to economical mass-production. The sensors utilize the sensitivity and surface chemistry of a 2D MoS2 transducer for attachment of antibody fragments in a conformation favorable for antigen binding with no need for additional linker molecules. To make the devices, ultra-thin layers (3 nm) of amorphous MoS2 are sputtered over pre-patterned metal electrical contacts on a glass chip at room temperature. The amorphous MoS2 is then laser annealed to create an array of semiconducting 2H-MoS2 transducer regions between metal contacts. The semiconducting crystalline MoS2 region is functionalized with monoclonal antibody fragments complementary to either SARS-CoV-2 S1 spike protein or influenza A hemagglutinin. Quartz crystal microbalance experiments indicate strong binding and maintenance of antigen avidity for antibody fragments bound to MoS2. Electrical resistance measurements of sensors exposed to antigen concentrations ranging from 2-20 000 pg mL(-1) reveal selective responses. Sensor architecture is adjusted to produce an array of sensors on a single chip suited for detection of analyte concentrations spanning six orders of magnitude from pg mL(-1) to mu g mL(-1).
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页数:9
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共 45 条
[1]   Laser writing of electronic circuitry in thin film molybdenum disulfide: A transformative manufacturing approach [J].
Austin, Drake ;
Gliebe, Kimberly ;
Muratore, Christopher ;
Boyer, Bryce ;
Fisher, Timothy S. ;
Beagle, Lucas K. ;
Benton, Anna ;
Look, Paige ;
Moore, David ;
Ringe, Emilie ;
Treml, Benjamin ;
Jawaid, Ali ;
Vaia, Richard ;
Kennedy, W. Joshua ;
Buskohl, Philip ;
Glavin, Nicholas R. .
MATERIALS TODAY, 2021, 43 (43) :17-26
[2]   Influenza hemagglutinin membrane anchor [J].
Benton, Donald J. ;
Nans, Andrea ;
Calder, Lesley J. ;
Turner, Jack ;
Neu, Ursula ;
Lin, Yi Pu ;
Ketelaars, Esther ;
Kallewaard, Nicole L. ;
Corti, Davide ;
Lanzavecchia, Antonio ;
Gamblin, Steven J. ;
Rosenthal, Peter B. ;
Skehel, John J. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115 (40) :10112-10117
[4]   Opportunities and Challenges for Biosensors and Nanoscale Analytical Tools for Pandemics: COVID-19 [J].
Bhalla, Nikhil ;
Pan, Yuwei ;
Yang, Zhugen ;
Payam, Amir Farokh .
ACS NANO, 2020, 14 (07) :7783-7807
[5]   STRUCTURE OF INFLUENZA-VIRUS HEMAGGLUTININ COMPLEXED WITH A NEUTRALIZING ANTIBODY [J].
BIZEBARD, T ;
GIGANT, B ;
RIGOLET, P ;
RASMUSSEN, B ;
DIAT, O ;
BOSECKE, P ;
WHARTON, SA ;
SKEHEL, JJ ;
KNOSSOW, M .
NATURE, 1995, 376 (6535) :92-94
[6]   Two-Dimensional Materials in Biosensing and Healthcare: From In Vitro Diagnostics to Optogenetics and Beyond [J].
Bolotsky, Adam ;
Butler, Derrick ;
Dong, Chengye ;
Gerace, Katy ;
Glayin, Nicholas R. ;
Muratore, Christopher ;
Robinson, Joshua A. ;
Ebrahimi, Aida .
ACS NANO, 2019, 13 (09) :9781-9810
[7]   Functionalization of MoS2 with 1,2-dithiolanes: toward donor-acceptor nanohybrids for energy conversion [J].
Canton-Vitoria, Ruben ;
Sayed-Ahmad-Baraza, Yuman ;
Pelaez-Fernandez, Mario ;
Arenal, Raul ;
Bittencourt, Carla ;
Ewels, Christopher P. ;
Tagmatarchis, Nikos .
NPJ 2D MATERIALS AND APPLICATIONS, 2017, 1
[8]   Nanotechnologies for biomolecular detection and medical diagnostics [J].
Cheng, MMC ;
Cuda, G ;
Bunimovich, YL ;
Gaspari, M ;
Heath, JR ;
Hill, HD ;
Mirkin, CA ;
Nijdam, AJ ;
Terracciano, R ;
Thundat, T ;
Ferrari, M .
CURRENT OPINION IN CHEMICAL BIOLOGY, 2006, 10 (01) :11-19
[9]   Synthesis and characterization of MoS2 nanosheets [J].
Deokar, G. ;
Vignaud, D. ;
Arenal, R. ;
Louette, P. ;
Colomer, J-F .
NANOTECHNOLOGY, 2016, 27 (07)
[10]   Biorecognition Layer Engineering: Overcoming Screening Limitations of Nanowire-Based FET Devices [J].
Elnathan, Roey ;
Kwiat, Moria ;
Pevzner, Alexander ;
Engel, Yoni ;
Burstein, Larisa ;
Khatchtourints, Artium ;
Lichtenstein, Arnir ;
Kantaev, Raisa ;
Patolsky, Fernando .
NANO LETTERS, 2012, 12 (10) :5245-5254