Effect of chitosan on indirubin production from suspension culture of Polygonum tinctorium

被引:21
作者
Kim, JH
Shin, JH
Lee, HJ
Chung, IS
Lee, HJ
机构
[1] SEOUL NATL UNIV,DEPT FOOD SCI & TECHNOL,SUWON 441744,SOUTH KOREA
[2] DONGEUI TECH JR COLL,DEPT FOOD TECHNOL,PUSAN 614715,SOUTH KOREA
[3] KYUNG HEE UNIV,DEPT GENET ENGN,SUWON 449701,SOUTH KOREA
来源
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1997年 / 83卷 / 02期
关键词
chitosan; indirubin; Polygonum tinctorium; elicitation;
D O I
10.1016/S0922-338X(97)83585-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effect of chitosan on indirubin production by Polygonum tinctorium cells cultured in shake flasks was investigated. The optimum concentration of chitosan for indirubin production was found to be 200 mg/l, at which the specific indirubin concentration (indirubin concentration per unit weight of cells) was 5.17 mg/g dry cell weight (DCW). The optimum period of elicitation was 5 d in the presence of chitosan. Indirubin production was enhanced by 72% when the cells were cultured in the presence of 200 mg/l of chitosan for 5 d using SH medium supplemented with 2.72 mM calcium chloride and 5 mM indole.
引用
收藏
页码:206 / 208
页数:3
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