Active conformation of alpha-chymotrypsin in organic solvents as studied by circular dichroism

被引：17

作者：

Sasaki, T ^{[1
]}

Kobayashi, M ^{[1
]}

Kise, H ^{[1
]}

机构：

[1] UNIV TSUKUBA,INST MAT SCI,TSUKUBA,IBARAKI 305,JAPAN

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 06期

关键词：

D O I：

10.1023/A:1018456420407

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Circular dichroic (CD) spectra of alpha-chymotrypsin were measured in mixtures of water and ethanol, acetonitrile, or tetrahydrofuran. The catalytic activity of alpha-chymotrypsin in the different solvents was related to the change of CD bands especially that at 230 nm. The fine structure in near-UV region may also be useful to estimate the catalytic activity of alpha-chymotrypsin.

引用

页码：387 / 390

页数：4

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