The isolation and antioxidant activity of polysaccharides from the marine microalgae Isochrysis galbana

被引:113
作者
Sun Yingying [1 ]
Wang Hui [2 ]
Guo Ganlin [1 ]
Pu Yinfang [1 ]
Yan Binlun [1 ]
机构
[1] Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China
[2] Beijing Inst Med Device Testing, Beijing 101111, Peoples R China
关键词
Antioxidant activities; Isochrysis galbana; Isolation; Polysaccharides; OXIDATIVE STRESS; RED ALGA; EXTRACTS; FRACTIONS; SPECTROSCOPY; DERIVATIVES; RAMAN;
D O I
10.1016/j.carbpol.2014.06.058
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Three polysaccharides, IPSI-A, IPSI-B and IFSII, were successfully isolated from the marine microalgae Isochrysis galbana through a combination of anion-exchange column chromatography and repeated gel chromatography. These three polysaccharides were demonstrated to have moderate scavenging activities against superoxide and hydroxyl radicals and moderate reductive power in a concentration-dependent manner. The IPSII demonstrated more effective antioxidant activities than IPSI-A and IPSI-B. IPSII had a molecular weight of 15.934 kDa belonging to a beta-type heteropolysaccharide with a pyran group and primarily contained mannose with variable amounts of glucose, galactose and rhamnose based on an analysis of infrared spectroscopy (IR), electrospray ionization-mass spectrometry (ESI-MS) and H-1 nuclear magnetic resonance (H-1 NMR). (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:22 / 31
页数:10
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