Efficient and controllable synthesis of highly substituted gelatin methacrylamide for mechanically stiff hydrogels

被引：134

作者：

Lee, Bae Hoon ^{[1
]}

Shirahama, Hitomi ^{[1
]}

Cho, Nam-Joon ^{[1
,2
]}

Tan, Lay Poh ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Mat Sci & Engn, Singapore 639798, Singapore

[2] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore 637459, Singapore

来源：

RSC ADVANCES | 2015年 / 5卷 / 128期

基金：

新加坡国家研究基金会;

关键词：

CROSS-LINKING; PHOTOCROSSLINKABLE GELATIN; FUNCTIONALIZED GELATIN; NETWORKS; MATRIX; BIOINK; FILMS;

D O I：

10.1039/c5ra22028a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report an effective and novel method to controllably produce highly substituted gelatin-MA with nearly 100% degree of substitution despite the use of a very low concentration of methacrylic anhydride (MAA). The method is based on sequential time-lapse loading of MAA after pH adjustment in a carbonate-bicarbonate buffer system. Rheological studies indicate that hydrogels formed from more highly substituted gelatin-MA exhibit significantly improved mechanical stiffness.

引用

页码：106094 / 106097

页数：4

共 30 条

[1] Stabilization of gelatin films by crosslinking with genipin [J].

Bigi, A ;

Cojazzi, G ;

Panzavolta, S ;

Roveri, N ;

Rubini, K .

BIOMATERIALS, 2002, 23 (24) :4827-4832

[2] Mechanical and thermal properties of gelatin films at different degrees of glutaraldehyde crosslinking [J].

Bigi, A ;

Cojazzi, G ;

Panzavolta, S ;

Rubini, K ;

Roveri, N .

BIOMATERIALS, 2001, 22 (08) :763-768

[3] Quantitative Contrasts in the Photopolymerization of Acrylamide and Methacrylamide-Functionalized Gelatin Hydrogel Building Blocks [J].

Billiet, Thomas ;

Van Gasse, Bjorn ;

Gevaert, Elien ;

Cornelissen, Maria ;

Martins, Jose C. ;

Dubruel, Peter .

MACROMOLECULAR BIOSCIENCE, 2013, 13 (11) :1531-1545

[4] The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability [J].

Billiet, Thomas ;

Gevaert, Elien ;

De Schryver, Thomas ;

Cornelissen, Maria ;

Dubruel, Peter .

BIOMATERIALS, 2014, 35 (01) :49-62

[5] Functional Human Vascular Network Generated in Photocrosslinkable Gelatin Methacrylate Hydrogels [J].

Chen, Ying-Chieh ;

Lin, Ruei-Zeng ;

Qi, Hao ;

Yang, Yunzhi ;

Bae, Hojae ;

Melero-Martin, Juan M. ;

Khademhosseini, Ali .

ADVANCED FUNCTIONAL MATERIALS, 2012, 22 (10) :2027-2039

[6] Interpenetrating networks based on gelatin methacrylamide and PEG formed using concurrent thiol click chemistries for hydrogel tissue engineering scaffolds [J].

Daniele, Michael A. ;

Adams, Andre A. ;

Naciri, Jawad ;

North, Stella H. ;

Ligler, Frances S. .

BIOMATERIALS, 2014, 35 (06) :1845-1856

[7] Improvement of Hydrogelation Abilities and Handling of Photocurable Gelatin-based Crosslinking Materials [J].

Fukaya, Chie ;

Nakayama, Yasuhide ;

Murayama, Yoshinobu ;

Omata, Sadao ;

Ishikawa, Ayaka ;

Hosaka, Yasuo ;

Nakagawa, Taneaki .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2009, 91B (01) :329-336

[8] Chemical tailoring of gelatin to adjust its chemical and physical properties for functional bioprinting [J].

Hoch, Eva ;

Hirth, Thomas ;

Tovar, Guenter E. M. ;

Borchers, Kirsten .

JOURNAL OF MATERIALS CHEMISTRY B, 2013, 1 (41) :5675-5685

[9] Stiff gelatin hydrogels can be photo-chemically synthesized from low viscous gelatin solutions using molecularly functionalized gelatin with a high degree of methacrylation [J].

Hoch, Eva ;

Schuh, Christian ;

Hirth, Thomas ;

Tovar, Guenter E. M. ;

Borchers, Kirsten .

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2012, 23 (11) :2607-2617

[10] Synthesis and Characterization of Tunable Poly(Ethylene Glycol): Gelatin Methacrylate Composite Hydrogels [J].

Hutson, Che B. ;

Nichol, Jason W. ;

Aubin, Hug ;

Bae, Hojae ;

Yamanlar, Seda ;

Al-Haque, Shahed ;

Koshy, Sandeep T. ;

Khademhosseini, Ali .

TISSUE ENGINEERING PART A, 2011, 17 (13-14) :1713-1723

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