Molecular characterization of HOXC8 gene and methylation status analysis of its exon 1 associated with the length of cashmere fiber in Liaoning cashmere goat

被引：20

作者：

Bai, Wen L. ^{[1
]}

Wang, Jiao J. ^{[1
]}

Yin, Rong H. ^{[1
]}

Dang, Yun L. ^{[1
]}

Wang, Ze Y. ^{[1
]}

Zhu, Yu B. ^{[1
]}

Cong, Yu Y. ^{[1
]}

Deng, Liang ^{[1
]}

Guo, Dan ^{[2
]}

Wang, Shi Q. ^{[2
]}

Yang, Shu H. ^{[2
]}

Xue, Hui L. ^{[1
]}

机构：

[1] Shenyang Agr Univ, Coll Anim Sci & Vet Med, Key Lab Zoonosis Liaoning Prov, Shenyang 110866, Peoples R China

[2] Inst Anim Husb Sci Liaoning Prov, Liaoyang 130062, Peoples R China

来源：

GENETICA | 2017年 / 145卷 / 01期

基金：

中国国家自然科学基金;

关键词：

HOXC8; gene; Secondary hair follicle; Genetic evolution; Methylation; Liaoning cashmere goat; HAIR FOLLICLE ACTIVITY; 3-DIMENSIONAL STRUCTURES; DIFFERENTIAL EXPRESSION; DNA METHYLATION; SKIN TISSUE; 3RD HELIX; IDENTIFICATION; RECOGNITION; GROWTH; ERRORS;

D O I：

10.1007/s10709-017-9950-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Homeobox protein Hox-C8 (HOXC8) is a member of Hox family. It is expressed in the dermal papilla of the skin and is thought to be associated with the hair inductive capacity of dermal papilla cells. In the present study, we isolated and characterized a full-length open reading frame of HOXC8 cDNA from the skin tissue of Liaoning cashmere goat, as well as, established a phylogenetic relationship of goat HOXC8 with that of other species. Also, we investigated the effect of methylation status of HOXC8 exon 1 at anagen secondary hair follicle on the cashmere fiber traits in Liaoning cashmere goat. The sequence analysis indicated that the obtained cDNA was 1134-bp in length containing a complete ORF of 729-bp. It encoded a peptide of 242 amino acid residues in length. The structural analysis indicated that goat HOXC8 contained a typical homeobox domain. The phylogenetic analysis revealed that Capra hircus HOXC8 had a closer genetic relationship with that of Ovis aries, followed by Bos Taurus and Bubalus bubalis. The methylation analysis suggested that the methylation degree of HOXC8 exon 1 in anagen secondary hair follicle might be involved in regulating the growth of cashmere fiber in Liaoning cashmere goat. Our results provide new evidence for understanding the molecular structural and evolutionary characteristics of HOXC8 in Liaoning cashmere goat, as well as, for further insight into the role of methylation degree of HOXC8 exon 1 regulates the growth of cashmere fiber in goat.

引用

页码：115 / 126

页数：12

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