First Molecular Detection of Babesia ovis, Theileria spp., Anaplasma spp., and Ehrlichia ruminantium in Goats from Western Uganda

被引：23

作者：

Tumwebaze, Maria Agnes ^{[1
,2
]}

Byamukama, Benedicto ^{[1
,2
]}

Tayebwa, Dickson Stuart ^{[2
,3
]}

Byaruhanga, Joseph ^{[2
]}

Angwe, Martin Kamilo ^{[2
]}

Galon, Eloiza May ^{[1
]}

Liu, Mingming ^{[1
]}

Lee, Seung-Hun ^{[1
,4
]}

Ringo, Aaron Edmond ^{[1
,5
]}

Adjou Moumouni, Paul Franck ^{[1
]}

Li, Jixu ^{[1
]}

Li, Yongchang ^{[1
]}

Ji, Shengwei ^{[1
]}

Vudriko, Patrick ^{[2
,3
]}

Xuan, Xuenan ^{[1
]}

机构：

[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan

[2] Makerere Univ, Coll Vet Med Anim Resources & Biosecur, Res Ctr Trop Dis & Vector Control, Kampala 7062, Uganda

[3] Makerere Univ, Coll Vet Med Anim Resources & Biosecur, Sch Vet Med & Anim Resources, Dept Vet Pharm Clin & Comparat Med, Kampala 7062, Uganda

[4] Chungbuk Natl Univ, Coll Vet Med, Cheongju 28644, South Korea

[5] Zanzibar Livestock Res Inst, Minist Agr, Nat Resources Livestock & Fisheries, POB 159, Zanzibar, Tanzania

来源：

PATHOGENS | 2020年 / 9卷 / 11期

基金：

日本学术振兴会;

关键词：

Babesia; Theileria; Anaplasma; Ehrlichia; goats; Uganda; TICK-BORNE PROTOZOAN; RICKETTSIAL PATHOGENS; DOMESTIC RUMINANTS; INFECTING SHEEP; NATIONAL-PARK; RISK-FACTORS; CATTLE; IDENTIFICATION; PREVALENCE; WILD;

D O I：

10.3390/pathogens9110895

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ticks and tick-borne diseases are major impediments to livestock production. To date, there have been several studies on the prevalence of tick-borne pathogens (TBPs) in cattle, but very few studies have documented TBPs in goats in Uganda. In this study, polymerase chain reaction assays and sequence analysis of different molecular markers were used to assess the presence and genetic characteristics of TBPs in 201 goats from Kasese district in western Uganda. The risk factors associated with TBP infections were also analyzed. We detected Theileria spp. (13.4%), Anaplasma phagocytophilum (10.9%), Anaplasma ovis (5.5%), Babesia ovis (5.5%), and Ehrlichia ruminantium (0.5%). The sequences of B. ovis ssu rRNA and A. ovis msp4 genes showed some degree of diversity among the parasite isolates in this study. The E. ruminantium pCS20 sequence formed a well-supported clade with isolates from Amblyomma variegatum ticks from Uganda. Wildlife interaction, sampling location, low body condition score, tick infestation, and herd size were significantly associated with TBP infections in the goats. The findings in this study provide important information on the epidemiology of tick-borne pathogens in Uganda, and show that goats could be potential reservoirs for tick-borne pathogens.

引用

页码：1 / 16

页数：16

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