Diagnosis of basal cell carcinoma by two photon excited fluorescence combined with lifetime imaging

被引：3

作者：

Fan, Shunping ^{[1
]}

Peng, Xiao ^{[1
]}

Liu, Lixin ^{[2
]}

Liu, Shaoxiong ^{[3
]}

Lu, Yuan ^{[3
]}

Qu, Junle ^{[1
]}

机构：

[1] Shenzhen Univ, Coll Optoelect Engn, Key Lab Optoelect Devices & Syst, Minist Educ & Guangdong Prov, Shenzhen 518060, Guangdong, Peoples R China

[2] Xidian Univ, Sch Phys & Optoelect Engn, Xian 710071, Peoples R China

[3] Sixth Peoples Hosp, Dept Dermatol & Pathol, Shenzhen 518054, Peoples R China

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XIV | 2014年 / 8948卷

基金：

中国国家自然科学基金;

关键词：

skin cancer; basal cell carcinoma; two-photon excited fluorescence; autofluorescence; fluorescence lifetime imaging microscopy (FLIM); MULTIPHOTON MICROSCOPY; SPECTROSCOPY; TOMOGRAPHY; GUIDELINES; EXCITATION; MANAGEMENT;

D O I：

10.1117/12.2038464

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Basal cell carcinoma (BCC) is the most common type of human skin cancer. The traditional diagnostic procedure of BCC is histological examination with haematoxylin and eosin staining of the tissue biopsy. In order to reduce complexity of the diagnosis procedure, a number of noninvasive optical methods have been applied in skin examination, for example, multiphoton tomography (MPT) and fluorescence lifetime imaging microscopy (FLIM). In this study, we explored two-photon optical tomography of human skin specimens using two-photon excited autofluorescence imaging and FLIM. There are a number of naturally endogenous fluorophores in skin sample, such as keratin, melanin, collagen, elastin, flavin and porphyrin. Confocal microscopy was used to obtain structures of the sample. Properties of epidermic and cancer cells were characterized by fluorescence emission spectra, as well as fluorescence lifetime imaging. Our results show that two-photon autofluorescence lifetime imaging can provide accurate optical biopsies with subcellular resolution and is potentially a quantitative optical diagnostic method in skin cancer diagnosis.

引用

页数：7

共 24 条

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