PHENOLIC PROFILE OF Centaurea aegyptiaca L. GROWING IN EGYPT AND ITS CYTOTOXIC AND ANTIVIRAL ACTIVITIES

被引:29
作者
Bakr, Riham Omar [1 ]
Mohamed, Shaza Abd El Halim [2 ]
Ayoub, Nahla [3 ]
机构
[1] October Univ Modern Sci & Arts MSA, Fac Pharm, Dept Pharmacognosy, Cairo, Egypt
[2] Al Azhar Univ Girls, Fac Pharm, Dept Pharmacognosy, Cairo, Egypt
[3] British Univ Egypt BUE, Fac Pharm, Dept Pharmacognosy, Cairo, Egypt
关键词
Centaurea aegyptiaca; Cytotoxicity; MTT assay; flavonoid; SESQUITERPENE LACTONES; ANTIOXIDANT ACTIVITY; MASS-SPECTROMETRY; AERIAL PARTS; FLAVONOIDS; MS; EXTRACT; LEAVES; PLANTS; ACIDS;
D O I
10.21010/ajtcam.v13i6.19
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Background: Centaurea aegyptiaca L (Asteraceae), is one of the most attractive plants growing wildly in Sinai, and is not well investigated for its phytochemical constituents. This study represents the first in-depth characterization of the phenolic profile of the aerial parts of C. aegyptiaca methanolic extract utilizing liquid chromatography (LC) combined with electrospray ionization (ESI) tandem mass spectrometry (MS/MS). Material and Methods: Phenolic profile was researched utilizing LC-HRESI-MS-MS. Assessment of cytotoxic activity against four human cancer cell lines (Hep-G2; hepatocellular carcinoma cells, MCF-7; breast adenocarcinoma cells, and HCT-116; colon carcinoma and HELA; cervical carcinoma cells) was performed using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Antiviral activity was surveyed utilizing cytopathic effect inhibition assay. Results: A total of sixty-one compounds were tentatively distinguished (twenty-one phenolic acids and their derivatives, thirty-one flavonols and nine flavones) in the negative and positive modes. Centaurea aegyptiaca demonstrated outstanding results against Hep-G2, MCF-7, HCT-116 and HELA cell lines with IC50 of 12.1, 30.9, 11.7 and 19.5 mu g/mL respectively compared and doxorubicin as a reference drug. Weak antiviral activity was seen against hepatitis A virus (HAV) and no impact against herpes simplex virus type 1 (HSV 1). Conclusion: This study provides a better understanding of the chemistry of C. aegyptiaca that announces itself as a promising cytotoxic agent.
引用
收藏
页码:135 / 143
页数:9
相关论文
共 40 条
[1]   Cytotoxic Activities of Flavonoids from Centaurea scoparia [J].
Ahmed, Sayed A. ;
Kamel, Emadeldin M. .
SCIENTIFIC WORLD JOURNAL, 2014,
[2]   Flavonoids from Centaurea furfuracea (Asteraceae) [J].
Akkal, S ;
Benayache, F ;
Medjroubi, K ;
Tillequin, F ;
Seguin, E .
BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2003, 31 (06) :641-643
[3]  
[Anonymous], J PHARM SCI
[4]   Comprehensive Phenolic Profiling of Cyclopia genistoides (L.) Vent. by LC-DAD-MS and -MS/MS Reveals Novel Xanthone and Benzophenone Constituents [J].
Beelders, Theresa ;
de Beer, Dalene ;
Stander, Maria A. ;
Joubert, Elizabeth .
MOLECULES, 2014, 19 (08) :11760-11790
[5]  
Boik J., 2001, NATURAL COMPOUNDS CA
[6]   HPLC-UV-MS Profiles of Phenolic Compounds and Antioxidant Activity of Fruits from Three Citrus Species Consumed in Northern Chile [J].
Brito, Anghel ;
Ramirez, Javier E. ;
Areche, Carlos ;
Sepulveda, Beatriz ;
Simirgiotis, Mario J. .
MOLECULES, 2014, 19 (11) :17400-17421
[7]   Determination of Phenolic Acids and Flavonoids in Taraxacum formosanum Kitam by Liquid Chromatography-Tandem Mass Spectrometry Coupled with a Post-Column Derivatization Technique [J].
Chen, Hung-Ju ;
Inbaraj, Baskaran Stephen ;
Chen, Bing-Huei .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2012, 13 (01) :260-285
[8]   Profiling the chlorogenic acids of aster by HPLC-MSn [J].
Clifford, Michael N. ;
Zheng, Wang ;
Kuhnert, Nikolai .
PHYTOCHEMICAL ANALYSIS, 2006, 17 (06) :384-393
[9]  
Cuyckens F, 2004, J MASS SPECTROM, V39, P1, DOI [10.1002/jms.585, 10.1002/jms.622]
[10]  
Dargan DJ, 1998, METH MOLEC MED, V10, P387, DOI 10.1385/0-89603-347-3:387