Proteomics Analysis of Alfalfa Response to Heat Stress

被引:42
作者
Li, Weimin [1 ]
Wei, Zhenwu [1 ]
Qiao, Zhihong [1 ]
Wu, Zinian [2 ]
Cheng, Lixiang [3 ]
Wang, Yuyang [4 ]
机构
[1] Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou, Peoples R China
[2] Chinese Acad Agr Sci, Inst Grassland Res, Hohhot, Peoples R China
[3] Gansu Agr Univ, Gansu Prov Key Lab Aridland Crop Sci, Res & Testing Ctr, Lanzhou, Peoples R China
[4] Yangzhou Univ, Testing Ctr, Yangzhou, Peoples R China
来源
PLOS ONE | 2013年 / 8卷 / 12期
基金
中国国家自然科学基金;
关键词
OXIDATIVE STRESS; HIGH-TEMPERATURE; RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE; DEHYDROASCORBATE REDUCTASE; GENE-EXPRESSION; SHOCK PROTEINS; DNA-BINDING; TOLERANCE; DROUGHT; FRUIT;
D O I
10.1371/journal.pone.0082725
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The proteome responses to heat stress have not been well understood. In this study, alfalfa (Medicago sativa L. cv. Huaiyin) seedlings were exposed to 25 degrees C (control) and 40 degrees C (heat stress) in growth chambers, and leaves were collected at 24, 48 and 72 h after treatment, respectively. The morphological, physiological and proteomic processes were negatively affected under heat stress. Proteins were extracted and separated by two-dimensional polyacrylamide gel electrophoresis (2-DE), and differentially expressed protein spots were identified by mass spectrometry (MS). Totally, 81 differentially expressed proteins were identified successfully by MALDI-TOF/TOF. These proteins were categorized into nine classes: including metabolism, energy, protein synthesis, protein destination/storage, transporters, intracellular traffic, cell structure, signal transduction and disease/defence. Five proteins were further analyzed for mRNA levels. The results of the proteomics analyses provide a better understanding of the molecular basis of heat-stress responses in alfalfa.
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页数:11
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