Development of novel amperometric urea biosensor based on Fc-PAMAM and MWCNT bio-nanocomposite film

被引：39

作者：

Dervisevic, Esma ^{[1
]}

Dervisevic, Muamer ^{[1
]}

Nyangwebah, Jackson Narkpa ^{[2
]}

Senel, Mehmet ^{[3
]}

机构：

[1] Mehmed Pase Sokolovica 21, Bihac 77000, Bosnia & Herceg

[2] Univ Liberia, Dept Biol Sci, TJR Coll Sci & Technol, Monrovia, Liberia

[3] EMC Technol Inc, Biotechnol Res Lab, ARGEM Bldg, TR-34320 Istanbul, Turkey

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2017年 / 246卷

关键词：

Ferrocence; PAMAM dendrimer; Urea; Urease; Bionanocomposite; Amperometric biosensor; MWCNT; WALLED CARBON NANOTUBES; ELECTROCHEMICAL BIOSENSOR; AMMONIUM ION; URICASE; DENDRIMERS; NANOPARTICLES; ELECTRODE; OXIDE; RECOGNITION; DERIVATIVES;

D O I：

10.1016/j.snb.2017.02.122

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, fabrication of new electrochemical urea biosensor based on ferrocene-poly(amidoamine) (Fc-PAMAM) dendrimers incorporated with multi walled carbon nanotubes (MWCNTs) is reported. Bio-sensing electrode was characterized using cyclic voltammogram where optimization studies were performed for reusability, storage, interference behaviors together with optimum temperature and pH parameters. Electrode showed excellent performance in urea detection with wide linear range of 0.2-1.8 mM, detection limit of 0.05 mM, fast response of 3 s, and sensitivity of 1.085 mu A/cm(2)/mM. Final assessment of electrochemical biosensor was real sample application which was performed in healthy human blood sample where excellent performance in selectivity and sensitivity of the bio-electrode was observed. Fabricated bio-sensing electrode has great potential in urea measurement applications. (C) 2017 Published by Elsevier B.V.

引用

页码：920 / 926

页数：7

共 48 条

[1] FERMENTATION OF CANDIDA-UTILIS FOR URICASE PRODUCTION [J].

ADAMEK, V ;

SUCHOVA, M ;

DEMNEROVA, K ;

KRALOVA, B ;

FORT, I ;

MORAVA, P .

JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1990, 6 (02) :85-89

[2]

Alkire R.C., 2012, ADV ELECTROCHEMICAL, P1, DOI [10.1002/9783527644117.ch1, DOI 10.1002/9783527644117.CH1]

[3] Recent Progress in the Synthesis and Applications of Some Ferrocene Derivatives and Ferrocene-Based Polymers [J].

Amer, Wael A. ;

Wang, Li ;

Amin, Abid M. ;

Ma, Liang ;

Yu, Haojie .

JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS, 2010, 20 (04) :605-615

[4] Electrical properties and applications of carbon nanotube structures [J].

Bandaru, Prabhakar R. .

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2007, 7 (4-5) :1239-1267

[5] Amperometric ammonium ion and urea determination with enzyme-based probes [J].

Bertocchi, P ;

Compagnone, D ;

Palleschi, G .

BIOSENSORS & BIOELECTRONICS, 1996, 11 (1-2) :1-10

[6] Reduced graphene oxide-carboxymethylcellulose layered with platinum nanoparticles/PAMAM dendrimer/magnetic nanoparticles hybrids. Application to the preparation of enzyme electrochemical biosensors [J].

Borisova, Boryana ;

Sanchez, Alfredo ;

Jimenez-Falcao, Sandra ;

Martin, Miriam ;

Salazar, Pedro ;

Parrado, Concepcion ;

Pingarron, Jose M. ;

Villalonga, Reynaldo .

SENSORS AND ACTUATORS B-CHEMICAL, 2016, 232 :84-90

[7]

Busono P., 2015, ADV SCI ENG MED, V7, P906, DOI [10.1166/asem.2015.1785, DOI 10.HTTP://DX.D0I.0RG/10.1166/ASEM.2015.1785]

[8] Detection of aflatoxin B1 by aptamer-based biosensor using PAMAM dendrimers as immobilization platform [J].

Castillo, Gabriela ;

Spinella, Katia ;

Poturnayova, Alexandra ;

Snejdarkova, Maja ;

Mosiello, Lucia ;

Hianik, Tibor .

FOOD CONTROL, 2015, 52 :9-18

[9] Potentiometric urea biosensor based on poly(glycidylmethacrylate)-grafted iron oxide nanoparticles [J].

Cevik, Emre ;

Senel, Mehmet ;

Baykal, Abdulhadi .

CURRENT APPLIED PHYSICS, 2013, 13 (01) :280-286

[10] Mediated biosensors [J].

Chaubey, A ;

Malhotra, BD .

BIOSENSORS & BIOELECTRONICS, 2002, 17 (6-7) :441-456

← 1 2 3 4 5 →