Establishment of mouse erythroleukemia cell lines expressing complete Influenza C virus CM2 protein or chimeric protein consisting of CM2 and Influenza A virus M2

被引：3

作者：

Muraki, Y. ^{[1
]}

Hay, A. ^{[1
]}

机构：

[1] Natl Inst Med Res, Div Virol, London NW7 1AA, England

来源：

ACTA VIROLOGICA | 2009年 / 53卷 / 02期

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

Influenza C virus; Influenza A virus; CM2; protein; chimeric protein; MEL cells; RNA SEGMENT 6; CYTOPLASMIC TAIL; PH; PHOSPHORYLATION; IDENTIFICATION; GLYCOPROTEIN; M-2;

D O I：

10.4149/av_2009_02_125

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The role of the Influenza C virus (ICV) CM2 protein in virus replication as well as its precise function as all ion channel remains to be elucidated. For this purpose, we established a CM2-expression, mouse erythroleukemia (MEL) cell line and determined the biochemical characteristics of the expressed CM2. The features of the expressed CM2 were similar to those of the viral CM2 synthesized in ICV-infected cells Furthermore, we established MEL cell line expressing a chimeric protein consisting of characteristic regions of CM2 and Influenza A virus (IAV) M2 Protein that could be helpful in elucidation of the specific ion conductance properties.

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页码：125 / 129

页数：5

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