An isogenic hemoglobin receptor-deficient mutant of Haemophilus ducreyi is attenuated in the human model of experimental infection

被引:65
作者
Al-Tawfiq, JA
Fortney, KR
Katz, BP
Hood, AF
Elkins, C
Spinola, SM
机构
[1] Indiana Univ, Sch Med, Dept Med, Indianapolis, IN 46202 USA
[2] Indiana Univ, Sch Med, Dept Microbiol & Immunol, Indianapolis, IN 46202 USA
[3] Indiana Univ, Sch Med, Dept Pathol & Lab Med, Indianapolis, IN 46202 USA
[4] Indiana Univ, Sch Med, Dept Dermatol, Indianapolis, IN 46202 USA
[5] Univ N Carolina, Sch Med, Dept Med, Chapel Hill, NC USA
关键词
D O I
10.1086/315309
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Haemophilus ducreyi expresses a conserved hemoglobin-binding outer-membrane protein (HgbA). To test the role of HgbA in pathogenesis, we infected 9 adults with isolate 35000 and its isogenic hgbA-inactivated mutant (FX504) on their upper arms in a double-blinded, escalating dose-response study. Papules developed at similar rates at sites inoculated with the mutant or parent. The pustule-formation rate was 55% (95% confidence interval [CI], 30.8%-78.5%) at parent sites and 0 (95% CI, 0-10.5%) at mutant sites(P < .0001). The recovery rate of H. ducreyi from surface cultures was 16% (n = 142) from parent sites and 0 (n = 213) from mutant sites (P < .0001). H. ducreyi was recovered at biopsy from 6 of 7 parent sites and from 0 of 3 mutant sites. The results indicate that hemoglobin may be a critical source of heme or iron for the establishment of H. ducreyi infection in humans.
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收藏
页码:1049 / 1054
页数:6
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