Micro-Nanostructured Protein Arrays: A Tool for Geometrically Controlled Ligand Presentation

被引：35
作者：
Aydin, Daniel [1 ,2 ]
Schwieder, Marco [1 ,2 ]
Louban, Ilia [1 ,2 ]
Knoppe, Stefan [1 ,2 ]
Ulmer, Jens [1 ,2 ]
Haas, Tobias L. [3 ]
Walczak, Henning [4 ]
Spatz, Joachim P. [1 ,2 ]
机构：
[1] Univ Heidelberg, Abt Neue Mat & Biosyst, Max Planck Inst Met Forsch, D-70569 Stuttgart, Germany
[2] Univ Heidelberg, Inst Biophys Chem, D-70569 Stuttgart, Germany
[3] Fdn Ist Oncol Mediterraneo, Viagrande, CT, Italy
[4] Univ London Imperial Coll Sci Technol & Med, Tumour Immunol Unit, Div Med, London W12 0NN, England
来源：
SMALL | 2009年 / 5卷 / 09期
关键词：
gold; lithography; nanoparticles; scanning force microscopy; DIP-PEN NANOLITHOGRAPHY; DIBLOCK COPOLYMER MICELLES; TAGGED PROTEINS; INTERFACES; LITHOGRAPHY; NANOARRAYS; SURFACES; NANOPARTICLES; LAYERS;
D O I：
10.1002/smll.200801219
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The combination of block copolymer micelle nanolithography (BCMN) with standard electron-beam lithography (EBL) and photolithography for rapid patterning of gold nanoparticles on two different length scales was presented. Cut silicon (110) chips and standard glass overslips were used as solid supports, while the formed miceller monolayer was treated with reactive hydrogen plasma upon evaporation of the solvent. The substrates are processed to yield coverslip-sized areas of micro-nanostructres with a patch size of 3 μm realizing the rapid parallel fabrication of high-quality micro-nanostructured surfaces with relatively low material and equipment cost. Only the nanostructured parts of the surface are covered by histidine-tagged GFP, proving the full preservation of protein repellent properties of the coupled PEG after the particle modification process.
引用
收藏
页码：1014 / 1018
页数：5
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