Microfluidic in-reservoir pre-concentration using a buffer drain technique

被引：9

作者：

Yoon, Junghyo ^{[1
]}

Cho, Youngkyu ^{[1
]}

Han, Sewoon ^{[1
]}

Lim, Chae Seung ^{[2
]}

Lee, Jeong Hoon ^{[3
]}

Chung, Seok ^{[1
]}

机构：

[1] Korea Univ, Sch Mech Engn, Seoul 136701, South Korea

[2] Korea Univ, Coll Med, Dept Lab Med, Seoul 136701, South Korea

[3] Kwangwoon Univ, Dept Elect Engn, Seoul 139701, South Korea

来源：

LAB ON A CHIP | 2014年 / 14卷 / 15期

基金：

新加坡国家研究基金会;

关键词：

SAMPLE STACKING;

D O I：

10.1039/c4lc00289j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Pre-concentration methods are essential for detecting low concentrations of influenza virus in biological samples from patients. Here, we describe a new method for draining buffer from solution in the reservoir of a microfluidic device to increase the concentration of virus in the reservoir. Viruses were captured in the reservoir by an ion depletion barrier from connected ion selective microfluidic channels. 75 mu l of buffer was successfully drained from a 100 mu l sample, resulting in a 4-fold increase in influenza hemagglutinin concentration in the reservoir. The volume of the final concentrated sample was suitable for detection of influenza hemagglutinin by the enzyme-linked immunosorbent assay, demonstrating the usefulness of the developed platform for enhanced sensitivity of virus detection in a conventional analysis.

引用

页码：2778 / 2782

页数：5

共 15 条

[1] OPTIMIZATION IN SAMPLE STACKING FOR HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS [J].