Use of segment-based microarray in the analysis of global gene expression in response to various environmental stresses in the cyanobacterium Anabaena sp PCC 7120

被引:20
作者
Sato, N
Ohmori, M
Ikeuchi, M
Tashiro, K
Wolk, CP
Kaneko, T
Okada, K
Tsuzuki, M
Ehira, S
Katoh, H
Okamoto, S
Yoshimura, H
Fujisawa, T
Kamei, A
Yoshihara, S
Narikawa, R
Hamano, T
Tabata, S
Kuhara, S
机构
[1] Saitama Univ, Fac Sci, Dept Biochem & Mol Biol, Sakura Ku, Saitama 3388570, Japan
[2] Univ Tokyo, Dept Life Sci Biol, Meguro Ku, Tokyo 1538902, Japan
[3] Kyushu Univ, Fac Agr, Dept Genet Resources Technol, Fukuoka 8128581, Japan
[4] Michigan State Univ, MSU DOE, Plant Res Lab, E Lansing, MI 48824 USA
[5] Kazusa DNA Res Inst, Chiba 2920818, Japan
[6] Tokyo Univ Pharm & Life Sci, Sch Life Sci, Tokyo 1920392, Japan
关键词
Anabaena; desiccation; genome-wide gene expression; low temperature; microarray; nitrogen deficiency;
D O I
10.2323/jgam.50.1
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We prepared microarrays that contain genomic sequences of a heterocyst-forming filamentous cyanobacterium Anabaena sp. PCC 7120. The complete genome of this cyanobacterium codes for about 5,368 protein-coding genes in the main chromosome of 6.4 Mbp. In total, 2,407 DNA segments were selected from the sequencing clones, and amplified by PCR, then spotted on glass slides in duplicate. These microarrays differ from the widely used commercial or custom-made ones for other microorganisms in that each DNA segment was 3-4 kbp long, and contained about 3-4 predicted genes on average. This feature, however, did not decrease the usefulness of the microarrays, since we were able to detect a number of potentially novel genes that are induced in response to nitrogen deprivation, low temperature and drought. In addition, we found some genomic regions in which dozens of contiguous genes are simultaneously regulated. These results suggest that these segment-based microarrays are useful especially for such large genomes as Anabaena, for which the number of genes exceeds either technical or practical limitations.
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页码:1 / 8
页数:8
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