Rapid Functional Maturation of Nascent Dendritic Spines

被引：192
作者：
Zito, Karen [1 ,3 ]
Scheuss, Volker [1 ,4 ]
Knott, Graham [2 ]
Hill, Travis [3 ]
Svoboda, Karel [1 ,5 ]
机构：
[1] Cold Spring Harbor Lab, Howard Hughes Med Inst, Cold Spring Harbor, NY 11724 USA
[2] Ecole Polytech Fed Lausanne, Interdisciplinary Ctr Electron Microscopy, CH-1015 Lausanne, Switzerland
[3] Univ Calif Davis, Ctr Neurosci, Davis, CA 95618 USA
[4] Max Planck Inst Neurobiol, Dept Cellular & Syst Neurobiol, D-82152 Martinsried, Germany
[5] Howard Hughes Med Inst, Ashburn, VA 20147 USA
来源：
NEURON | 2009年 / 61卷 / 02期
基金：
瑞士国家科学基金会;
关键词：
LONG-TERM POTENTIATION; POSTSYNAPTICALLY SILENT SYNAPSES; INDIVIDUAL EXCITATORY SYNAPSES; CORTEX IN-VIVO; NMDA RECEPTORS; HIPPOCAMPAL-NEURONS; SYNAPTIC PLASTICITY; IMMUNOGOLD LOCALIZATION; POSTNATAL-DEVELOPMENT; DEPENDENT PLASTICITY;
D O I：
10.1016/j.neuron.2008.10.054
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Spine growth and retraction with synapse formation and elimination plays an important role in shaping brain circuits during development and in the adult brain, yet the temporal relationship between spine morphogenesis and the formation of functional synapses remains poorly defined. We imaged hippocampal pyramidal neurons to identify spines of different ages. We then used two-photon glutamate uncaging, whole-cell recording, and Ca(2+), imaging to analyze the properties of nascent spines and their older neighbors. New spines expressed glutamate-sensitive currents that were indistinguishable from mature spines of comparable volumes. Some spines exhibited negligible AMPA receptor-mediated responses, but the occurrence of these "silent" spines was uncorrelated with spine age. In contrast, NMDA receptor-mediated Ca(2+) accumulations were significantly lower in new spines. New spines reconstructed using electron microscopy made synapses. Our data support a model in which outgrowth and enlargement of nascent spines is tightly coupled to formation and maturation of glutamatergic synapses.
引用
收藏
页码：247 / 258
页数：12
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