In vitro synergy of caspofungin with licensed and novel antifungal drugs against clinical isolates of Fusarium spp.

被引：16
作者：
Shalit, Itamar [2 ]
Shadkchan, Yona
Mircus, Gabriel
Osherov, Nir [1 ]
机构：
[1] Tel Aviv Univ, Sackler Sch Med, Dept Human Microbiol, IL-69978 Tel Aviv, Israel
[2] Schneider Childrens Med Ctr, Petah Tiqwa, Israel
来源：
MEDICAL MYCOLOGY | 2009年 / 47卷 / 05期
关键词：
Caspofungin; Fusarium; synergy; tacrolimus; terbinafine; AMPHOTERICIN-B; ASPERGILLUS; ITRACONAZOLE; VORICONAZOLE; CALCINEURIN; COMBINATION; INFECTIONS; RESOLUTION; THERAPY;
D O I：
10.1080/13693780802232910
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Combinations of caspofungin (CAS) with amphotericin-B (AMB), voriconazole (VRC), terbinafine (TRB) and tacrolimus (FK-506) were tested in vitro with 10 Fusarium isolates. MIC and minimal effective concentrations (MEC) were investigated in accord with the CLSI methodology. Drug interactions were assessed by the fractional inhibitory concentration index (FICI). Synergy occurred in 10/10, 9/10, 7/10 and 4/10 isolates with CAS/FK-506, CAS/TRB, CAS/AMB and CAS/VRC, respectively. Caspofungin MECs reached clinically attainable concentrations with FK-506 and TRB. Hyphal length and DiBAC staining demonstrated enhanced inhibition and killing with CAS/FK-506 and CAS/TRB. The combination of CAS/TRB and CAS/FK-506 is strongly synergistic in vitro against Fusarium spp. Our finding should be further studied in animal models of invasive infections caused by this fungus.
引用
收藏
页码：457 / 462
页数：6
相关论文
共 12 条
[1]   Resolution of Fungemia due to Fusarium species in a patient with acute leukemia treated with caspofungin [J].
Apostolidis, J ;
Bouzani, M ;
Platsouka, E ;
Belasiotou, H ;
Stamouli, M ;
Harhalakis, N ;
Boutati, EI ;
Paniara, O ;
Nikiforakis, E .
CLINICAL INFECTIOUS DISEASES, 2003, 36 (10) :1349-1350
[2]   In vitro synergy of caspofungin and amphotericin B against Aspergillus and Fusarium spp. [J].
Arikan, S ;
Lozano-Chiu, M ;
Paetznick, V ;
Rex, JH .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (01) :245-247
[3]   Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole [J].
Espinel-Ingroff, A ;
Fothergill, A ;
Ghannoum, M ;
Manavathu, E ;
Ostrosky-Zeichner, L ;
Pfaller, M ;
Rinaldi, M ;
Schell, W ;
Walsh, T .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (10) :5243-5246
[4]   Treating disseminated fusariosis: amphotericin B, voriconazole or both? [J].
Ho, Dora Y. ;
Lee, Jason D. ;
Rosso, Fernando ;
Montoya, Jose G. .
MYCOSES, 2007, 50 (03) :227-231
[5]   Combination of caspofungin with inhibitors of the calcineurin pathway attenuates growth in vitro in Aspergillus species [J].
Kontoyiannis, DP ;
Lewis, RE ;
Osherov, N ;
Albert, ND ;
May, GS .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2003, 51 (02) :313-316
[6]   Resolution of disseminated fusariosis in a child with acute leukemia treated with combined antifungal therapy: a case report [J].
Manuel Vagace, Jose ;
Sanz-Rodriguez, Cesar ;
Casado, Maria-Soledad ;
Alonso, Nieves ;
Garcia-Dominguez, Manuel ;
Garcia de la Llana, Francisco ;
Zarallo, Luis ;
Fajardo, Miguel ;
Bajo, Roberto .
BMC INFECTIOUS DISEASES, 2007, 7 (1)
[7]   Fusarium Infections in Immunocompromised Patients [J].
Nucci, Marcio ;
Anaissie, Elias .
CLINICAL MICROBIOLOGY REVIEWS, 2007, 20 (04) :695-704
[8]   In vitro interactions of licensed and novel antifungal drugs against Fusarium spp [J].
Ortoneda, M ;
Capilla, J ;
Pastor, FJ ;
Pujol, I ;
Guarro, J .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2004, 48 (01) :69-71
[9]   Combination therapy of disseminated Fusarium oxysporum infection with terbinafine and amphotericin B [J].
Rothe, A ;
Seibold, M ;
Hoppe, T ;
Seifert, H ;
Engert, A ;
Caspar, C ;
Karthaus, M ;
Fätkenheuer, G ;
Bethe, U ;
Tintelnot, K ;
Cornely, OA .
ANNALS OF HEMATOLOGY, 2004, 83 (06) :394-397
[10]   In vitro synergy of caspofungin and itraconazole against Aspergillus spp.:: MIC versus minimal effective concentration end points [J].
Shalit, I ;
Shadkchan, Y ;
Samra, Z ;
Osherov, N .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (04) :1416-1418
12