Insulin stimulates the entry of GLUT4 into the endosomal recycling pathway by a quantal mechanism

被引:51
|
作者
Coster, ACF [1 ]
Govers, R
James, DE
机构
[1] Univ New S Wales, Sch Math, Sydney, NSW 2052, Australia
[2] Garvan Inst Med Res, Diabet & Obes Res Program, Sydney, NSW 2010, Australia
关键词
exocytosis; kinetics; modelling; secretion; vesicle transport;
D O I
10.1111/j.1600-0854.2004.00218.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The insulin-sensitive glucose transporter GLUT4 mediates the uptake of glucose into adipocytes and muscle cells. In this study we have used a novel 96-well plate fluorescence assay to study the kinetics of GLUT4 trafficking in 3T3-L1 adipocytes. We have found evidence for a graded release mechanism whereby GLUT4 is released into the plasma membrane recycling system in a nonkinetic manner as follows: the kinetics of appearance of GLUT4 at the plasma membrane is independent of the insulin concentration; a large proportion of GLUT4 molecules do not participate in plasma membrane recycling in the absence of insulin; and with increasing insulin there is an incremental increase in the total number of GLUT4 molecules participating in the recycling pathway rather than simply an increased rate of recycling. We propose a model whereby GLUT4 is stored in a compartment that is disengaged from the plasma membrane recycling system in the basal state. In response to insulin, GLUT4 is quantally released from this compartment in a pulsatile manner, leaving some sequestered from the recycling pathway even in conditions of excess insulin. Once disengaged from this location we suggest that in the continuous presence of insulin this quanta of GLUT4 continuously recycles to the plasma membrane, possibly via non-endosomal carriers that are formed at the perinuclear region.
引用
收藏
页码:763 / 771
页数:9
相关论文
共 50 条
  • [1] Insulin responsiveness of endosomal GLUT4 vesicles revealed by a novel GLUT4 probe
    Chen, Y.
    Lippincott-Schwartz, J.
    MOLECULAR BIOLOGY OF THE CELL, 2013, 24
  • [2] Insulin increases cell surface GLUT4 levels by dose dependently discharging GLUT4 into a cell surface recycling pathway
    Govers, R
    Coster, ACF
    James, DE
    MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (14) : 6456 - 6466
  • [3] Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin -stimulated sarcolemmal and endosomal GLUT4 translocation
    Knudsen, Jonas R.
    Steenberg, Dorte E.
    Hingst, Janne R.
    Hodgson, Lorna R.
    Henriquez-Olguin, Carlos
    Li, Zhencheng
    Kiens, Bente
    Richter, Erik A.
    Wojtaszewski, Jorgen F. P.
    Verkade, Paul
    Jensen, Thomas E.
    MOLECULAR METABOLISM, 2020, 39
  • [4] Mechanism and regulation of cargo entry into the Commander endosomal recycling pathway
    Butkovic, Rebeka
    Walker, Alexander P.
    Healy, Michael D.
    McNally, Kerrie E.
    Liu, Meihan
    Veenendaal, Tineke
    Kato, Kohji
    Liv, Nalan
    Klumperman, Judith
    Collins, Brett M.
    Cullen, Peter J.
    NATURE COMMUNICATIONS, 2024, 15 (01)
  • [5] Insulin Mobilizes Peripheral Endosomal GLUT4 Compartments to the Plasma Membrane
    Chen, Yu
    Lippincott-Schwartz, Jennifer
    BIOPHYSICAL JOURNAL, 2013, 104 (02) : 651A - 651A
  • [6] Insulin stimulates translocation of both GLUT4 and GLUT1 in the heart
    Russell, RR
    Yin, FR
    Hu, XY
    Dione, D
    Ren, JM
    Shulman, GI
    Sinusas, AJ
    Caplan, MJ
    Young, LH
    CIRCULATION, 1996, 94 (08) : 1794 - 1794
  • [7] Bradykinin stimulates GLUT4 translocation through the enhancement of insulin receptor signaling pathway.
    Araki, E
    Isami, S
    Kishikawa, H
    Uehara, M
    Shichiri, M
    DIABETES, 1996, 45 : 1151 - 1151
  • [8] Insulin-induced GLUT4 recycling in rat adipose cells by a pathway insensitive to brefeldin A
    KonoSugita, E
    Satoh, S
    Suzuki, Y
    Egawa, M
    Udaka, N
    Ito, T
    Sekihara, H
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 236 (03): : 1033 - 1037
  • [9] Insulin stimulates GLUT4 translocation in the semitendinosus muscle of Shetland ponies
    Duehlmeier, Reinhard
    Hacker, Anja
    Widdel-Bigdely, Andrea
    von Engelhardt, Wolfgang
    Sallmann, Hans-Peter
    VETERINARY JOURNAL, 2010, 184 (02): : 176 - 181
  • [10] Intracellular insulin-responsive glucose transporter (GLUT4) distribution but not insulin-stimulated GLUT4 exocytosis and recycling are microtubule dependent
    Shigematsu, S
    Khan, AH
    Kanzaki, M
    Pessin, JE
    MOLECULAR ENDOCRINOLOGY, 2002, 16 (05) : 1060 - 1068