M2, M3, and M4 muscarinic receptors are expressed in the guinea pig gallbladder

被引：2
|
作者：
Cabadak, Hulya [1 ]
Cuadra, Adolfo E. [2 ]
El-Fakahany, Esam E. [2 ]
Kan, Beki [1 ]
机构：
[1] Marmara Univ, Sch Med, Dept Biophys, TR-34668 Istanbul, Turkey
[2] Univ Minnesota, Sch Med, Div Neurosci Res Psychiat, Minneapolis, MN 55455 USA
来源：
JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION | 2009年 / 29卷 / 01期
基金：
美国国家卫生研究院;
关键词：
Muscarinic receptors; guinea pig gallbladder; RT-PCR; MESSENGER-RNA LEVELS; SMOOTH-MUSCLE; CELLS;
D O I：
10.1080/10799890802687416
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Aim: The identity of muscarinic acetylcholine receptors (mAchR) involved in cholinergic-mediated contraction of the guinea pig gallbladder has been a matter of debate. Different groups have suggested the involvement of M-1, M-2, M-3, or M-4 receptor subtypes in the contraction of this tissue. The objective of this study was to identify the mAchR subtypes expressed in the guinea pig gallbladder by RT-PCR. Methods: Total RNA prepared from frozen guinea pig gallbladder tissue was amplified by using specific primers for the M-1-M-4 receptor subtypes. Results: M-2, M-3, and M-4 transcripts were detected in the following rank order: M-4 > M-2 > M-3. We were unable to demonstrate the expression of the M, receptor subtype in this tissue. Conclusions: Our results are in agreement with our previous binding and functional data.
引用
收藏
页码：63 / 66
页数：4
相关论文
共 50 条
  • [31] A MUSCARINIC RECEPTOR DIFFERENT FROM THE M1, M2, M3 AND M4 SUBTYPES MEDIATES THE CONTRACTION OF THE RABBIT IRIS SPHINCTER
    BOGNAR, IT
    ALTES, U
    BEINHAUER, C
    KESSLER, I
    FUDER, H
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 1992, 345 (06) : 611 - 618
  • [32] Development of muscarinic m3 and m4 receptor antibodies with pharmacological activities
    王海鹰
    曾淑君
    邱鹏新
    ActaPharmacologicaSinica, 1998, (06) : 24 - 27
  • [33] Acetylcholine induces Ca2+ oscillations via m3/m4 muscarinic receptors in the mouse oocyte
    Kang, D
    Park, JY
    Han, J
    Bae, IH
    Yoon, SY
    Kang, SS
    Choi, WS
    Hong, SG
    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2003, 447 (03): : 321 - 327
  • [34] Development of muscarinic m3 and m4 receptor antibodies with pharmacological activities
    Wang, HY
    Zeng, SJ
    Qiu, PX
    ACTA PHARMACOLOGICA SINICA, 1998, 19 (06): : 523 - 526
  • [35] Acetylcholine induces Ca2+ oscillations via m3/m4 muscarinic receptors in the mouse oocyte
    Dawon Kang
    Jae-Yong Park
    Jaehee Han
    In-Ha Bae
    Sook-Young Yoon
    Sang Soo Kang
    Wan Sung Choi
    Seong-Geun Hong
    Pflügers Archiv, 2003, 447 : 321 - 327
  • [36] A role for M2 and M3 muscarinic receptors in the contraction of rat and human small airways
    Brown, Stephanie M.
    Koarai, Akira
    Sturton, Richard G.
    Nicholson, Andrew G.
    Barnes, Peter J.
    Donnelly, Louise E.
    EUROPEAN JOURNAL OF PHARMACOLOGY, 2013, 702 (1-3) : 109 - 115
  • [37] Discussion:: Functional role of M1, M2, and M3 muscarinic receptors in overactive bladder
    Igawa, Y
    UROLOGY, 2000, 55 (5A) : 47 - 49
  • [38] Pharmacological analysis of the contractile role of M2 and M3 muscarinic receptors in smooth muscle
    Ehlert, FJ
    RECEPTORS & CHANNELS, 2003, 9 (04): : 261 - 277
  • [39] Differential agonist-induced regulation of human M2 and M3 muscarinic receptors
    Stope, MB
    Kunkel, C
    Kories, C
    Schmidt, M
    Michel, MC
    BIOCHEMICAL PHARMACOLOGY, 2003, 66 (11) : 2099 - 2105
  • [40] Localization of M2 and M3 muscarinic receptors in human bladder disorders and their clinical correlations
    Mukerji, Gaurav
    Yiangou, Yiangos
    Grogono, Joanna
    Underwood, Jenny
    Agarwal, Sanjiv K.
    Khullar, Vikram
    Anand, Praveen
    JOURNAL OF UROLOGY, 2006, 176 (01): : 367 - 373
12345