Monochromosomal Hybrids and Chromosome Transfer: A Functional Approach for Gene Identification

被引：3

作者：

Kandpal, Raj P. ^{[1
]}

Sandhu, Arbans K. ^{[2
]}

Kaur, Gurpreet ^{[2
]}

Kaur, Gursurinder P. ^{[2
]}

Athwal, Raghbir S. ^{[2
]}

机构：

[1] Western Univ Hlth Sci, Dept Basic Med Sci, 309 E 2nd St, Pomona, CA 91766 USA

[2] Temple Univ, Sch Med, Fels Inst Canc Res & Mol Biol, Philadelphia, PA USA

来源：

CANCER GENOMICS & PROTEOMICS | 2017年 / 14卷 / 02期

关键词：

Chromosome transfer; monochromosomal hybrids; functional cloning; cell senescence; senescence associated beta-galactosidase; glutamate ionotropic receptor kainate type subunit 2; GRIK-2; GluR6; protein tyrosine phosphatase; receptor type K (PTPRK); review; SOMATIC-CELL HYBRIDS; ISOLATED METAPHASE CHROMOSOMES; XERODERMA-PIGMENTOSUM-CELLS; MICROCELL-MEDIATED TRANSFER; DNA-REPAIR DEFECT; MAMMALIAN-CELLS; MOUSE CELLS; ARTIFICIAL CHROMOSOME; CHINESE-HAMSTER; TUMOR-CELLS;

D O I：

10.21873/cgp.20022

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Functional complementation of cellular defects has been a valuable approach for localizing causative genes to specific chromosomes. The complementation strategy was followed by positional cloning and characterization of genes for their biological relevance. We herein describe strategies used for the construction of monochromosomal hybrids and their applications for cloning and characterization of genes related to cell growth, cell senescence and DNA repair. We have cloned RNaseT2, GluR6 (glutamate ionotropic receptor kainate type subunit 2-GRIK2) and protein tyrosine phosphatase, receptor type K (PTPRK) genes using these strategies.

引用

页码：93 / 101

页数：9

共 77 条

[61] LINKAGE RELATIONSHIPS OF 17 HUMAN GENE LOCI AS DETERMINED BY MAN-MOUSE SOMATIC CELL HYBRIDS [J].

RUDDLE, FH ;

CHAPMAN, VM ;

RICCIUTI, F ;

MURNANE, M ;

KLEBE, R ;

KHAN, PM .

NATURE-NEW BIOLOGY, 1971, 232 (29) :69-&

[62] SENESCENCE OF IMMORTAL HUMAN FIBROBLASTS BY THE INTRODUCTION OF NORMAL HUMAN-CHROMOSOME-6 [J].

SANDHU, AK ;

HUBBARD, K ;

KAUR, GP ;

JHA, KK ;

OZER, HL ;

ATHWAL, RS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (12) :5498-5502

[63]

Sandhu AK, 1996, ONCOGENE, V12, P247

[64] A MONOCHROMOSOMAL HYBRID CELL ASSAY FOR EVALUATING THE GENOTOXICITY OF ENVIRONMENTAL CHEMICALS [J].

SANDHU, SS ;

GUDI, RD ;

ATHWAL, RS .

CELL BIOLOGY AND TOXICOLOGY, 1988, 4 (04) :495-506

[65] MICROCELL-MEDIATED TRANSFER OF A SINGLE HUMAN-CHROMOSOME COMPLEMENTS XERODERMA-PIGMENTOSUM GROUP-A FIBROBLASTS [J].

SCHULTZ, RA ;

SAXON, PJ ;

GLOVER, TW ;

FRIEDBERG, EC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (12) :4176-4179

[66] CLONING AND STABLE MAINTENANCE OF 300-KILOBASE-PAIR FRAGMENTS OF HUMAN DNA IN ESCHERICHIA-COLI USING AN F-FACTOR-BASED VECTOR [J].

SHIZUYA, H ;

BIRREN, B ;

KIM, UJ ;

MANCINO, V ;

SLEPAK, T ;

TACHIIRI, Y ;

SIMON, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (18) :8794-8797

[67] TRANSFER OF CODOMINANT MARKERS BY ISOLATED METAPHASE CHROMOSOMES IN CHINESE-HAMSTER OVARY CELLS [J].

SPANDIDOS, DA ;

SIMINOVITCH, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) :3480-3484

[68] Highly Efficient Transfer of Chromosomes to a Broad Range of Target Cells Using Chinese Hamster Ovary Cells Expressing Murine Leukemia Virus-Derived Envelope Proteins [J].

Suzuki, Teruhiko ;

Kazuki, Yasuhiro ;

Oshimura, Mitsuo ;

Hara, Takahiko .

PLOS ONE, 2016, 11 (06)

[69] IMPROVED DERIVATIVE OF A PHAGE LAMBDA EK2 VECTOR FOR CLONING RECOMBINANT DNA [J].

TIEMEIER, D ;

ENQUIST, L ;

LEDER, P .

NATURE, 1976, 263 (5577) :526-527

[70] ASSIGNMENT OF GENE FOR ADENINE PHOSPHORIBOSYLTRANSFERASE TO HUMAN CHROMOSOME-16 BY MOUSE-HUMAN SOMATIC-CELL HYBRIDIZATION [J].

TISCHFIELD, JA ;

RUDDLE, FH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (01) :45-49

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