Monochromosomal Hybrids and Chromosome Transfer: A Functional Approach for Gene Identification

被引：3
作者：
Kandpal, Raj P. [1 ]
Sandhu, Arbans K. [2 ]
Kaur, Gurpreet [2 ]
Kaur, Gursurinder P. [2 ]
Athwal, Raghbir S. [2 ]
机构：
[1] Western Univ Hlth Sci, Dept Basic Med Sci, 309 E 2nd St, Pomona, CA 91766 USA
[2] Temple Univ, Sch Med, Fels Inst Canc Res & Mol Biol, Philadelphia, PA USA
来源：
CANCER GENOMICS & PROTEOMICS | 2017年 / 14卷 / 02期
关键词：
Chromosome transfer; monochromosomal hybrids; functional cloning; cell senescence; senescence associated beta-galactosidase; glutamate ionotropic receptor kainate type subunit 2; GRIK-2; GluR6; protein tyrosine phosphatase; receptor type K (PTPRK); review; SOMATIC-CELL HYBRIDS; ISOLATED METAPHASE CHROMOSOMES; XERODERMA-PIGMENTOSUM-CELLS; MICROCELL-MEDIATED TRANSFER; DNA-REPAIR DEFECT; MAMMALIAN-CELLS; MOUSE CELLS; ARTIFICIAL CHROMOSOME; CHINESE-HAMSTER; TUMOR-CELLS;
D O I：
10.21873/cgp.20022
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Functional complementation of cellular defects has been a valuable approach for localizing causative genes to specific chromosomes. The complementation strategy was followed by positional cloning and characterization of genes for their biological relevance. We herein describe strategies used for the construction of monochromosomal hybrids and their applications for cloning and characterization of genes related to cell growth, cell senescence and DNA repair. We have cloned RNaseT2, GluR6 (glutamate ionotropic receptor kainate type subunit 2-GRIK2) and protein tyrosine phosphatase, receptor type K (PTPRK) genes using these strategies.
引用
收藏
页码：93 / 101
页数：9
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